Calcium Alginate Gel As Encapsulation Matrix for Coimmobilized Enzyme Systems

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2003 Aug 12

PMID 12909732

Citations 7

Authors

A Blandino

M Macias

D Cantero

Affiliations

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Abstract

Encapsulation within calcium alginate gel capsules was used to produce a coimmobilized enzyme system. Glucose oxidase (GOD) and catalase (CAT) were chosen as model enzymes. The same values of Vmax and Km app for the GOD encapsulated system and for the GOD-CAT coencapsulated system were calculated. When gel beads and capsules were compared, the same catalyst deactivation sequence for the two enzymes was observed. However, when capsules were employed as immobilization support, GOD efficiencies were higher than for the gel beads. These results were explained in terms of the structure of the capsules.

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