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Detection of Methicillin-resistant Staphylococcus Aureus (MRSA) in Blood with the EVIGENE MRSA Detection Kit

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Specialty Microbiology
Date 2003 Aug 9
PMID 12904411
Citations 7
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Abstract

A total of 200 blood cultures containing putative staphylococci were analyzed by a commercial gene probe hybridization assay (EVIGENE; Statens Serum Institut, Copenhagen, Denmark), and 18 were identified as methicillin-resistant Staphylococcus aureus (MRSA) positive. Of these, 17 were positive by PCR and 16 were positive by culture. Detailed analysis of the discrepant results showed that the EVIGENE kit allowed specific identification of MRSA in blood cultures without any of the drawbacks associated with PCR.

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References
1.
Kempf V, Trebesius K, Autenrieth I . Fluorescent In situ hybridization allows rapid identification of microorganisms in blood cultures. J Clin Microbiol. 2000; 38(2):830-8. PMC: 86216. DOI: 10.1128/JCM.38.2.830-838.2000. View

2.
Skov R, Pallesen L, Poulsen R, Espersen F . Evaluation of a new 3-h hybridization method for detecting the mecA gene in Staphylococcus aureus and comparison with existing genotypic and phenotypic susceptibility testing methods. J Antimicrob Chemother. 1999; 43(4):467-75. DOI: 10.1093/jac/43.4.467. View

3.
Lem P, SPIEGELMAN J, Toye B, Ramotar K . Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles. Diagn Microbiol Infect Dis. 2001; 41(3):165-8. DOI: 10.1016/s0732-8893(01)00301-7. View

4.
Oliveira K, Procop G, Wilson D, Coull J, Stender H . Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes. J Clin Microbiol. 2002; 40(1):247-51. PMC: 120122. DOI: 10.1128/JCM.40.1.247-251.2002. View

5.
Tan T, Corden S, BARNES R, Cookson B . Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR. J Clin Microbiol. 2001; 39(12):4529-31. PMC: 88580. DOI: 10.1128/JCM.39.12.4529-4531.2001. View