Quantitative Real-time PCR Assay for Detection of Ehrlichia Chaffeensis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2003 Aug 9

PMID 12904406

Citations 20

Authors

Amanda D Loftis

Robert F Massung

Michael L Levin

Affiliations

Soon will be listed here.

Abstract

A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis. The assay is species specific and provides quantitative results in the range 10 to 10(10) gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis.

Citing Articles

Science abhors a surveillance vacuum: Detection of ticks and tick-borne pathogens in southern New Mexico through passive surveillance.

Harman P, Mendell N, Harman M, Draney P, Boyle A, Gompper M PLoS One. 2024; 19(1):e0292573.

PMID: 38295027 PMC: 10830002. DOI: 10.1371/journal.pone.0292573.

Release the hens: a study on the complexities of guinea fowl as tick control.

White A, Cumbie A, Brinkerhoff R, Hynes W, Gaff H J Med Entomol. 2024; 61(2):410-417.

PMID: 38279688 PMC: 11279834. DOI: 10.1093/jme/tjad167.

A community approach for pathogens and their arthropod vectors (ticks and fleas) in cats of sub-Saharan Africa.

Madder M, Day M, Schunack B, Fourie J, Labuschange M, van der Westhuizen W Parasit Vectors. 2022; 15(1):321.

PMID: 36085039 PMC: 9461260. DOI: 10.1186/s13071-022-05436-y.

Systematic evaluation of TaqMan real-time polymerase chain reaction assays targeting the and loci of in recombinant plasmids and naturally infected dogs.

Sumpavong P, Sricharern W, Inthong N, Kaewmongkol G, Kaewmongkol S Vet World. 2022; 15(3):701-706.

PMID: 35497947 PMC: 9047146. DOI: 10.14202/vetworld.2022.701-706.

A community approach of pathogens and their arthropod vectors (ticks and fleas) in dogs of African Sub-Sahara.

Heylen D, Day M, Schunack B, Fourie J, Labuschange M, Johnson S Parasit Vectors. 2021; 14(1):576.

PMID: 34784947 PMC: 8594167. DOI: 10.1186/s13071-021-05014-8.

References

Dumler J, Barbet A, Bekker C, Dasch G, Palmer G, Ray S . Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species.... Int J Syst Evol Microbiol. 2002; 51(Pt 6):2145-2165. DOI: 10.1099/00207713-51-6-2145. View

McQuiston J, Paddock C, Holman R, Childs J . The human ehrlichioses in the United States. Emerg Infect Dis. 1999; 5(5):635-42. PMC: 2627718. DOI: 10.3201/eid0505.990504. View

Massung R, Lee K, Mauel M, Gusa A . Characterization of the rRNA genes of Ehrlichia chaffeensis and Anaplasma phagocytophila. DNA Cell Biol. 2002; 21(8):587-96. DOI: 10.1089/104454902320308960. View

Weiss E, Dasch G, Kang Y, Westfall H . Substrate utilization by Ehrlichia sennetsu and Ehrlichia risticii separated from host constituents by renografin gradient centrifugation. J Bacteriol. 1988; 170(11):5012-7. PMC: 211565. DOI: 10.1128/jb.170.11.5012-5017.1988. View

DAWSON J, Rikihisa Y, Ewing S, Fishbein D . Serologic diagnosis of human ehrlichiosis using two Ehrlichia canis isolates. J Infect Dis. 1991; 163(3):564-7. DOI: 10.1093/infdis/163.3.564. View

DAWSON J, Anderson B, Fishbein D, Sanchez J, Goldsmith C, WILSON K . Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis. J Clin Microbiol. 1991; 29(12):2741-5. PMC: 270425. DOI: 10.1128/jcm.29.12.2741-2745.1991. View

Anderson B, Sumner J, DAWSON J, TZIANABOS T, Greene C, Olson J . Detection of the etiologic agent of human ehrlichiosis by polymerase chain reaction. J Clin Microbiol. 1992; 30(4):775-80. PMC: 265160. DOI: 10.1128/jcm.30.4.775-780.1992. View

Anderson B, Sims K, Olson J, Childs J, Piesman J, Happ C . Amblyomma americanum: a potential vector of human ehrlichiosis. Am J Trop Med Hyg. 1993; 49(2):239-44. DOI: 10.4269/ajtmh.1993.49.239. View

Wen B, Rikihisa Y, Mott J, Fuerst P, Kawahara M, Suto C . Ehrlichia muris sp. nov., identified on the basis of 16S rRNA base sequences and serological, morphological, and biological characteristics. Int J Syst Bacteriol. 1995; 45(2):250-4. DOI: 10.1099/00207713-45-2-250. View

10.

Ewing S, DAWSON J, Kocan A, Barker R, Warner C, Panciera R . Experimental transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) among white-tailed deer by Amblyomma americanum (Acari: Ixodidae). J Med Entomol. 1995; 32(3):368-74. DOI: 10.1093/jmedent/32.3.368. View

11.

Yeh M, Mather T, Coughlin R, Sumner J, Massung R . Serologic and molecular detection of granulocytic ehrlichiosis in Rhode Island. J Clin Microbiol. 1997; 35(4):944-7. PMC: 229705. DOI: 10.1128/jcm.35.4.944-947.1997. View

12.

Paddock C, Sumner J, Shore G, Bartley D, Elie R, McQuade J . Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis. J Clin Microbiol. 1997; 35(10):2496-502. PMC: 229999. DOI: 10.1128/jcm.35.10.2496-2502.1997. View

13.

Lockhart J, Davidson W, Stallknecht D, DAWSON J, Little S . Natural history of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) in the piedmont physiographic province of Georgia. J Parasitol. 1997; 83(5):887-94. View

14.

Sumner J, Childs J, Paddock C . Molecular cloning and characterization of the Ehrlichia chaffeensis variable-length PCR target: an antigen-expressing gene that exhibits interstrain variation. J Clin Microbiol. 1999; 37(5):1447-53. PMC: 84798. DOI: 10.1128/JCM.37.5.1447-1453.1999. View

15.

Buller R, Arens M, Hmiel S, Paddock C, Sumner J, Rikhisa Y . Ehrlichia ewingii, a newly recognized agent of human ehrlichiosis. N Engl J Med. 1999; 341(3):148-55. DOI: 10.1056/NEJM199907153410303. View

16.

Li J, Chu F, Reilly A, Winslow G . Antibodies highly effective in SCID mice during infection by the intracellular bacterium Ehrlichia chaffeensis are of picomolar affinity and exhibit preferential epitope and isotype utilization. J Immunol. 2002; 169(3):1419-25. DOI: 10.4049/jimmunol.169.3.1419. View