Development of a Sensitive Gene Expression Reporter System and an Inducible Promoter-repressor System for Clostridium Acetobutylicum

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2003 Aug 7

PMID 12902297

Citations 20

Authors

Laurence Girbal

Isabelle Mortier-Barriere

Frederic Raynaud

Celine Rouanet

Christian Croux

Philippe Soucaille

Affiliations

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Abstract

A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of beta-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter expression in C. acetobutylicum. Furthermore, a new inducible gene expression system was developed in C. acetobutylicum, based on the Staphylococcus xylosus xylose operon promoter-repressor regulatory system.

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