Cloning and Characterization of Taenia Saginata Paramyosin CDNA

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2003 Aug 5

PMID 12898225

Citations 8

Authors

Elizabeth Ferrer

Eva Moyano

Laura Benitez

Luis Miguel Gonzalez

Denise Bryce

Mildred Foster-Cuevas

Iris Davila

Maria Milagros Cortez

Leslie J S Harrison

R Michael E Parkhouse

Teresa Garate

Affiliations

Soon will be listed here.

Abstract

A lambdaZAP-express cDNA library of Taenia saginata metacestodes was constructed. Antibody screening yielded a clone with an insert of 3,408 bp, an open reading frame of 2,589 bp, a deduced sequence of 863 amino acid and a molecular mass of 98.89 kDa. Alignments of the predicted amino acid sequence showed identity with paramyosins from several species: 98.8% with Taenia solium, 96.3% with Echinococcus.granulosus and about 70% with Schistosoma spp. The insert was expressed and purified. A collagen binding assay was performed which showed that T. saginata GST-paramyosin retained this property in a dose-dependent manner. Problems were encountered due to high backgrounds in serological assays in the homologous T. saginata system. However, the recombinant paramyosin was recognized by antibodies present in 31.6% of sera from T. solium seropositive cysticercosis patients and 100% of the sera from acute cysticercosis patients. The immunodominant epitope was the carboxyl-terminal fragment of the molecule.

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