Genetic and Physical Mapping of Sequence-specific Amplified Polymorphic (SSAP) Markers on the 1RS Chromosome Arm of Rye in a Wheat Background

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2003 Aug 5

PMID 12898027

Citations 11

Authors

E D Nagy

T Lelley

Affiliations

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Abstract

Three rye-specific repeated sequences, pSc10C, pSc20H and R173-1, were used to design sequence-specific anchored primers. These primers and 16 restriction site-specific adaptor primers were used in all possible combinations to establish sequence-specific amplified polymorphic (SSAP) markers for the 1RS chromosome arm of rye in a wheat background. Thirty 1RS-specific SSAP markers were detected in 19 primer combinations. Along with six markers localised previously on 1RS, 26 of the SSAP markers were mapped genetically in wheat genotypes carrying recombinant 1BL.1RS translocations. A clear decrease in recombination frequency from distal to proximal regions was observed. Wheat-rye addition lines for the 1R chromosome with different-sized deletions of the short arm were used to physically localise these markers. Physical mapping suggested an even distribution of the SSAP markers along the total length of the 1RS chromosome arm.

Citing Articles

Homoeologous relationship of rye chromosome arms as detected with wheat PLUG markers.

Li J, Endo T, Saito M, Ishikawa G, Nakamura T, Nasuda S Chromosoma. 2013; 122(6):555-64.

PMID: 23873186 DOI: 10.1007/s00412-013-0428-7.

Involvement of disperse repetitive sequences in wheat/rye genome adjustment.

Tomas D, Bento M, Viegas W, Silva M Int J Mol Sci. 2012; 13(7):8549-8561.

PMID: 22942719 PMC: 3430250. DOI: 10.3390/ijms13078549.

Sequence composition and gene content of the short arm of rye (Secale cereale) chromosome 1.

Fluch S, Kopecky D, Burg K, Simkova H, Taudien S, Petzold A PLoS One. 2012; 7(2):e30784.

PMID: 22328922 PMC: 3273464. DOI: 10.1371/journal.pone.0030784.

Development of a wheat genotype combining the recessive crossability alleles kr1kr1kr2kr2 and the 1BL.1RS translocation, for the rapid enrichment of 1RS with new allelic variation.

Molnar-Lang M, Cseh A, Szakacs E, Molnar I Theor Appl Genet. 2010; 120(8):1535-45.

PMID: 20145905 DOI: 10.1007/s00122-010-1274-0.

Genomic subtraction recovers rye-specific DNA elements enriched in the rye genome.

Tomita M, Akai K, Morimoto T Mol Biotechnol. 2009; 42(2):160-7.

PMID: 19288229 DOI: 10.1007/s12033-009-9151-2.

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