Conditional Suppression of Cellular Genes: Lentivirus Vector-mediated Drug-inducible RNA Interference

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2003 Jul 30

PMID 12885912

Citations 321

Authors

Maciej Wiznerowicz

Didier Trono

Affiliations

Soon will be listed here.

Abstract

RNA interference has emerged as a powerful technique to downregulate the expression of specific genes in cells and in animals, thus opening new perspectives in fields ranging from developmental genetics to molecular therapeutics. Here, we describe a method that significantly expands the potential of RNA interference by permitting the conditional suppression of genes in mammalian cells. Within a lentivirus vector background, we subjected the polymerase III promoter-dependent production of small interfering RNAs to doxycycline-controllable transcriptional repression. The resulting system can achieve the highly efficient and completely drug-inducible knockdown of cellular genes. As lentivirus vectors can stably transduce a wide variety of targets both in vitro and in vivo and can be used to generate transgenic animals, the present system should have broad applications.

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