» Articles » PMID: 12882918

Pancreatic Precursors and Differentiated Islet Cell Types from Murine Embryonic Stem Cells: an in Vitro Model to Study Islet Differentiation

Overview
Journal Diabetes
Specialty Endocrinology
Date 2003 Jul 29
PMID 12882918
Citations 36
Authors
Affiliations
Soon will be listed here.
Abstract

Embryonic stem (ES) cells differentiating in vitro reproduce many facets of early embryonic development, including the expression of developmentally regulated transcription factors and the differentiation of multipotent precursor cells. ES cells were evaluated for their ability to differentiate into pancreatic and islet lineage-restricted stages including pancreatic duodenal homeobox 1 (PDX1)-positive pancreatic precursor cells, early endocrine cell progenitors, and islet hormone-producing cells. Following growth and differentiation in nonselective medium containing serum, murine ES cells spontaneously differentiated into cells individually expressing each of the four major islet hormones: insulin, glucagon, somatostatin, and pancreatic polypeptide. PDX1 immunostaining cells appeared first, before hormone-positive cells had emerged. Hormone-positive cells appeared within focal clusters of cells coexpressing PDX1 and the nonclassical hormone markers peptide YY (YY) and islet amyloid polypeptide (IAPP) in combination with the definitive hormones, characteristic of endocrine cells appearing during early pancreaticogenesis. This system allows the investigation of many facets of islet development since it promotes the appearance of the complete range of islet phenotypes and reproduces important developmental stages of normal islet cytodifferentiation in differentiating ES cell cultures.

Citing Articles

Regenerative medicine: current research and perspective in pediatric surgery.

Deguchi K, Zambaiti E, De Coppi P Pediatr Surg Int. 2023; 39(1):167.

PMID: 37014468 PMC: 10073065. DOI: 10.1007/s00383-023-05438-6.


Expression profile of long non-coding RNA in inner Mongolian cashmere goat with putative roles in hair follicles development.

Ma R, Shang F, Rong Y, Pan J, Wang M, Niu S Front Vet Sci. 2022; 9:995604.

PMID: 36118352 PMC: 9478897. DOI: 10.3389/fvets.2022.995604.


Treatment of Diabetes Mellitus Using iPS Cells and Spice Polyphenols.

Ge Q, Chen L, Chen K J Diabetes Res. 2017; 2017:5837804.

PMID: 28758131 PMC: 5512026. DOI: 10.1155/2017/5837804.


Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions.

Jacobson E, Tzanakakis E J Biol Eng. 2017; 11:21.

PMID: 28680477 PMC: 5494890. DOI: 10.1186/s13036-017-0066-3.


Bone marrow derived stem cell therapy for type 2 diabetes mellitus.

Wehbe T, Abi Chahine N, Sissi S, Abou-Joaude I, Chalhoub L Stem Cell Investig. 2017; 3:87.

PMID: 28066789 PMC: 5182211. DOI: 10.21037/sci.2016.11.14.