Genetic Mapping of Dn7, a Rye Gene Conferring Resistance to the Russian Wheat Aphid in Wheat

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2003 Jul 25

PMID 12879254

Citations 9

Authors

Garret R Anderson

Dan Papa

Junhua Peng

M Tahir

Nora L V Lapitan

Affiliations

Soon will be listed here.

Abstract

The Russian wheat aphid is a significant pest problem in wheat and barley in North America. Genetic resistance in wheat is the most effective and economical means to control the damage caused by the aphid. Dn7 is a rye gene located on chromosome 1RS that confers resistance to the Russian wheat aphid. The gene was previously transferred from rye into a wheat background via a 1RS/1BL translocation. This study was conducted to genetically map Dn7 and to characterize the type of resistance the gene confers. The resistant line '94M370' was crossed with a susceptible wheat cultivar that also contains a pair of 1RS/1BL translocation chromosomes. The F(2) progeny from this cross segregated for resistance in a ratio of 3 resistant: 1 susceptible, indicating a single dominant gene. One-hundred and eleven RFLP markers previously mapped on wheat chromosomes 1A, 1B and 1D, barley chromosome 1H and rye chromosome 1R, were used to screen the parents for polymorphism. A genetic map containing six markers linked to Dn7, encompassing 28.2 cM, was constructed. The markers flanking Dn7 were Xbcd1434 and XksuD14, which mapped 1.4 cM and 7.4 cM from Dn7, respectively. Dn7 confers antixenosis, and provides a higher level of resistance than that provided by Dn4. The applications of Dn7 and the linked markers in wheat breeding are discussed.

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References

Friebe B, Heun M, Bushuk W . Cytological characterization, powdery mildew resistance and storage protein composition of tetraploid and hexaploid 1BL/1RS wheat-rye translocation lines. Theor Appl Genet. 2013; 78(3):425-32. DOI: 10.1007/BF00265307. View

Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G . Construction of an RFLP map of barley. Theor Appl Genet. 2013; 83(2):250-6. DOI: 10.1007/BF00226259. View

Causse M, Fulton T, Cho Y, Ahn S, Chunwongse J, Wu K . Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics. 1994; 138(4):1251-74. PMC: 1206261. DOI: 10.1093/genetics/138.4.1251. View

Baum M, Appels R . The cytogenetic and molecular architecture of chromosome 1R--one of the most widely utilized sources of alien chromatin in wheat varieties. Chromosoma. 1991; 101(1):1-10. DOI: 10.1007/BF00360680. View

Lander E, Green P, ABRAHAMSON J, Barlow A, Daly M, Lincoln S . MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987; 1(2):174-81. DOI: 10.1016/0888-7543(87)90010-3. View

Hull G, Halford N, Kreis M, Shewry P . Isolation and characterisation of genes encoding rye prolamins containing a highly repetitive sequence motif. Plant Mol Biol. 1991; 17(5):1111-5. DOI: 10.1007/BF00037153. View

Singh N, Shepherd K, McIntosh R . Linkage mapping of genes for resistance to leaf, stem and stripe rusts and ω-secalins on the short arm of rye chromosome 1R. Theor Appl Genet. 2013; 80(5):609-16. DOI: 10.1007/BF00224219. View

Liu M, Smith M, Gill S . Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6. Theor Appl Genet. 2003; 104(6-7):1042-1048. DOI: 10.1007/s00122-001-0831-y. View

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

10.

Deynze A, Nelson J, Sorrells M, McCouch S, Dubcovsky J, Dvorak J . Molecular-genetic maps for group 1 chromosomes of Triticeae species and their relation to chromosomes in rice and oat. Genome. 1995; 38(1):45-59. DOI: 10.1139/g95-006. View

11.

Devos K, Atkinson M, Chinoy C, Francis H, Harcourt R, Koebner R . Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet. 2013; 85(6-7):673-80. DOI: 10.1007/BF00225004. View

12.

Philipp U, Wehling P, Wricke G . A linkage map of rye. Theor Appl Genet. 2013; 88(2):243-8. DOI: 10.1007/BF00225904. View

13.

Kleinhofs A, Kilian A, Saghai Maroof M, Biyashev R, Hayes P, Chen F . A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet. 2013; 86(6):705-12. DOI: 10.1007/BF00222660. View

14.

Peng J, Korol A, Fahima T, Roder M, Ronin Y, Li Y . Molecular genetic maps in wild emmer wheat, Triticum dicoccoides: genome-wide coverage, massive negative interference, and putative quasi-linkage. Genome Res. 2000; 10(10):1509-31. PMC: 310947. DOI: 10.1101/gr.150300. View

15.

Devos K, Atkinson M, Chinoy C, Liu C, Gale M . RFLP-based genetic map of the homoeologous group 3 chromosomes of wheat and rye. Theor Appl Genet. 2013; 83(8):931-9. DOI: 10.1007/BF00232953. View

16.

Lawrence G, Shepherd K . Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet. 2013; 59(1):25-31. DOI: 10.1007/BF00275771. View

17.

Gabal M, Dimitri R . Humoral immunosuppressant activity of aflatoxin ingestion in rabbits measured by response to Mycobacterium bovis antigens using enzyme-linked immunosorbent assay and serum protein electrophoresis. Mycoses. 1998; 41(7-8):303-8. DOI: 10.1111/j.1439-0507.1998.tb00343.x. View