HPV 16 Detection in Cervical Lesions, Physical State of Viral DNA and Changes in P53 Gene

Overview

Journal Sao Paulo Med J

Specialty General Medicine

Date 2003 Jul 19

PMID 12870053

Citations 6

Authors

Ledy do Horto dos Santos Oliveira

Eliane de Vasconcelos Machado Rodrigues

Ana Paula Terra Alvim de Salles Lopes

Andre de Paula Fernandez

Silvia Maria Baeta Cavalcanti

Affiliations

Soon will be listed here.

Abstract

Context: Persistent infection with high risk human papillomavirus (HPV) has been linked to cervical carcinoma. Integration of viral DNA into host cell DNA is essential for this cancer development, promoting disruption of the HPV E2 gene, thus leading to unregulated increases in E6 and E7 proteins and inactivating the products of p53 and Rb tumor suppressor genes.

Objective: To investigate HPV 16 infection in cervical lesions, physical state of viral DNA and p53 gene alterations in a group of women attending a public health service.

Design: Prospective, non-controlled, transversal study.

Setting: Gynecological clinic of the School of Medicine, Universidade Federal Fluminense.

Sample: 43 consecutive patients with cervical lesions referred to our service.

Main Measurements: Cases were classified via cytology/histology as normal, HPV infection, condyloma, low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL) and carcinoma. HPV infection was studied via polymerase chain reaction (PCR) using two PCR primer sets, to determine DNA integration. p53 gene changes were investigated by single-strand conformation polymorphism (SSCP) analysis.

Results: One normal case, 7 HPV infections, 6 condylomas, 7 LSIL, 14 HSIL and 8 cancers were found, with 95% positive for HPV genome when tested using both L1 and E6 primers. HPV 16 was most prevalent (73.1%). HPV 16 DNA was integrated within the host genome in 3 LSIL. One LSIL progressed to HSIL by 13 months after first diagnosis. Among HPV 16-positive HSIL cases, 50% contained integrated viral DNA. HPV 16 E2 gene disruption was seen in 7 cancers (87.5%). Only smal-cell carcinoma showed intact HPV 16 E2 gene. Abnormal p53 bands detected by PCR/SSCP were observed in 4 cases: 2 squamous carcinoma with parametrium (exon 8) and two cervical intraepithelial neoplasia (CIN) III (exons 5 and 7). All cases presented HPV 16 E2 gene loss.

Conclusions: The sample had a high rate of high-risk HPV detected in benign and malignant lesions; high cervical cancer burden; HPV 16 DNA integration in all except one case of cancer; p53 gene changes in CIN III and in invasive cancer cases associated with DNA integration.

Citing Articles

Differences in the mutation of the p53 gene in exons 6 and 7 in cervical samples from HIV- and HPV-infected women.

Souza R, Gimenes F, de Abreu A, Rocha-Brischiliari S, de Carvalho M, Ferreira E Infect Agent Cancer. 2013; 8(1):38.

PMID: 24098975 PMC: 3851445. DOI: 10.1186/1750-9378-8-38.

Diversity and uncommon HPV types in HIV seropositive and seronegative women attending an STI clinic.

de Mattos A, de Freitas L, Lima B, Miranda A, Cruz Spano L Braz J Microbiol. 2013; 42(2):786-93.

PMID: 24031694 PMC: 3769843. DOI: 10.1590/S1517-838220110002000047.

A review of methods for detect human Papillomavirus infection.

Abreu A, Souza R, Gimenes F, Consolaro M Virol J. 2012; 9:262.

PMID: 23131123 PMC: 3507852. DOI: 10.1186/1743-422X-9-262.

Type-specific HPV prevalence in cervical cancer and high-grade lesions in Latin America and the Caribbean: systematic review and meta-analysis.

Ciapponi A, Bardach A, Glujovsky D, Gibbons L, Picconi M PLoS One. 2011; 6(10):e25493.

PMID: 21991313 PMC: 3186785. DOI: 10.1371/journal.pone.0025493.

Human papillomavirus in women with and without HIV-1 infection attending an STI clinic in Vitoria, Brazil.

Lima B, Golub J, Tonani Mattos A, de Freitas L, Cruz Spano L, Miranda A J Int Assoc Physicians AIDS Care (Chic). 2009; 8(5):286-90.

PMID: 19721099 PMC: 6198318. DOI: 10.1177/1545109709341855.

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