Identification of an Epitope of SARS-coronavirus Nucleocapsid Protein

Overview

Journal Cell Res

Specialty Cell Biology

Date 2003 Jul 17

PMID 12862314

Citations 56

Authors

Ying Lin

Xu Shen

Rui Fu Yang

Yi Xue Li

Yong Yong Ji

You Yu He

Mu De Shi

Wei Lu

Tie Liu Shi

Jin Wang

Hong Xia Wang

Hua Liang Jiang

Jian Hua Shen

You Hua Xie

Yuan Wang

Gang Pei

Bei Fen Shen

Jia Rui Wu

Bing Sun

Affiliations

Soon will be listed here.

Abstract

The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV were predicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodies were isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-induced polyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, it was confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratory syndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified and N protein specific Abs were produced by peptide immunization, which will be usefull for the study of SARS-CoV.

Citing Articles

SARS-CoV-2 and Brain Health: New Challenges in the Era of the Pandemic.

Ahmed W, Feng J, Zhang Y, Chen L Microorganisms. 2023; 11(10).

PMID: 37894169 PMC: 10609574. DOI: 10.3390/microorganisms11102511.

A gold nanoparticles-based lateral flow assay utilizing baculovirus expressed recombinant nucleocapsid and receptor binding domain proteins for serodetection of IgG and IgM against SARS-CoV-2.

Salem R, Elshamy A, Kamel N, Younes S, Marie O, Waly F Biotechnol Lett. 2022; 44(12):1507-1517.

PMID: 36319880 PMC: 9628349. DOI: 10.1007/s10529-022-03316-0.

Characterization and application of a series of monoclonal antibodies against SARS-CoV-2 nucleocapsid protein.

Zhou L, Mo C, Yang Y, Zhou Z, You A, Fan Y J Med Virol. 2022; 95(1):e28225.

PMID: 36238992 PMC: 9874425. DOI: 10.1002/jmv.28225.

Molecular adaptations during viral epidemics.

Rochman N, Wolf Y, Koonin E EMBO Rep. 2022; 23(8):e55393.

PMID: 35848484 PMC: 9346483. DOI: 10.15252/embr.202255393.

Peptides for Vaccine Development.

Hamley I ACS Appl Bio Mater. 2022; 5(3):905-944.

PMID: 35195008 PMC: 9384901. DOI: 10.1021/acsabm.1c01238.

References

Hiscox J, Wurm T, Wilson L, Britton P, Cavanagh D, Brooks G . The coronavirus infectious bronchitis virus nucleoprotein localizes to the nucleolus. J Virol. 2000; 75(1):506-12. PMC: 113943. DOI: 10.1128/JVI.75.1.506-512.2001. View

Liu C, Kokuho T, Kubota T, Watanabe S, Inumaru S, Yokomizo Y . DNA mediated immunization with encoding the nucleoprotein gene of porcine transmissible gastroenteritis virus. Virus Res. 2001; 80(1-2):75-82. DOI: 10.1016/s0168-1702(01)00333-1. View

Wu Y, Lin Y, Hou W, Wang Y, Sun B . IFN-gamma up-regulation of IL-12 beta 2 receptor is associated with experimental autoimmune uveitis susceptible B10.A mice, but not in EAU resistant BALB/c mice. J Neuroimmunol. 2003; 137(1-2):154-63. DOI: 10.1016/s0165-5728(03)00071-7. View

Holmes K, Enjuanes L . Virology. The SARS coronavirus: a postgenomic era. Science. 2003; 300(5624):1377-8. DOI: 10.1126/science.1086418. View

Yu L, Liu W, Schnitzlein W, Tripathy D, Kwang J . Study of protection by recombinant fowl poxvirus expressing C-terminal nucleocapsid protein of infectious bronchitis virus against challenge. Avian Dis. 2001; 45(2):340-8. View

Kuo L, Masters P . Genetic evidence for a structural interaction between the carboxy termini of the membrane and nucleocapsid proteins of mouse hepatitis virus. J Virol. 2002; 76(10):4987-99. PMC: 136159. DOI: 10.1128/jvi.76.10.4987-4999.2002. View

Rota P, Oberste M, Monroe S, Nix W, Campagnoli R, Icenogle J . Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science. 2003; 300(5624):1394-9. DOI: 10.1126/science.1085952. View

Narayanan K, Chen C, Maeda J, Makino S . Nucleocapsid-independent specific viral RNA packaging via viral envelope protein and viral RNA signal. J Virol. 2003; 77(5):2922-7. PMC: 149775. DOI: 10.1128/jvi.77.5.2922-2927.2003. View

Marra M, Jones S, Astell C, Holt R, Brooks-Wilson A, Butterfield Y . The Genome sequence of the SARS-associated coronavirus. Science. 2003; 300(5624):1399-404. DOI: 10.1126/science.1085953. View

10.

Ksiazek T, Erdman D, Goldsmith C, Zaki S, Peret T, Emery S . A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med. 2003; 348(20):1953-66. DOI: 10.1056/NEJMoa030781. View

11.

Lipsitch M, Cohen T, Cooper B, Robins J, Ma S, James L . Transmission dynamics and control of severe acute respiratory syndrome. Science. 2003; 300(5627):1966-70. PMC: 2760158. DOI: 10.1126/science.1086616. View

12.

Wu Y, Wang Y, Zhong C, Li Y, Li X, Sun B . The suppressive effect of triptolide on experimental autoimmune uveoretinitis by down-regulating Th1-type response. Int Immunopharmacol. 2003; 3(10-11):1457-65. DOI: 10.1016/S1567-5769(03)00144-9. View

13.

Riley S, Fraser C, Donnelly C, Ghani A, Abu-Raddad L, Hedley A . Transmission dynamics of the etiological agent of SARS in Hong Kong: impact of public health interventions. Science. 2003; 300(5627):1961-6. DOI: 10.1126/science.1086478. View

14.

Hou W, Wu Y, Sun S, Shi M, Sun Y, Yang C . Pertussis toxin enhances Th1 responses by stimulation of dendritic cells. J Immunol. 2003; 170(4):1728-36. DOI: 10.4049/jimmunol.170.4.1728. View

15.

Bloom B . Lessons from SARS. Science. 2003; 300(5620):701. DOI: 10.1126/science.300.5620.701. View