Phosphate Starvation Triggers Distinct Alterations of Genome Expression in Arabidopsis Roots and Leaves

Overview

Journal Plant Physiol

Specialty Physiology

Date 2003 Jul 15

PMID 12857808

Citations 186

Authors

Ping Wu

Ligeng Ma

Xingliang Hou

Mingyi Wang

Yungrong Wu

Feiyan Liu

Xing Wang Deng

Affiliations

Soon will be listed here.

Abstract

Arabidopsis genome expression pattern changes in response to phosphate (Pi) starvation were examined during a 3-d period after removal of Pi from the growth medium. Available Pi concentration was decreased after the first 24 h of Pi starvation in roots by about 22%, followed by a slow recovery during the 2nd and 3rd d after Pi starvation, but no significant change was observed in leaves within the 3 d of Pi starvation. Microarray analysis revealed that more than 1,800 of the 6,172 genes present in the array were regulated by 2-fold or more within 72 h from the onset of Pi starvation. Analysis of these Pi starvation-responsive genes shows that they belong to wide range of functional categories. Many genes for photosynthesis and nitrogen assimilation were down-regulated. A complex set of metabolic adaptations appears to occur during Pi starvation. More than 100 genes each for transcription factors and cell-signaling proteins were regulated in response to Pi starvation, implying major regulatory changes in cellular growth and development. A significant fraction of those regulatory genes exhibited distinct or even contrasting expression in leaves and roots in response to Pi starvation, supporting the idea that distinct Pi starvation response strategies are used for different plant organs in response to a shortage of Pi in the growth medium.

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References

Rubio V, LINHARES F, Solano R, Martin A, Iglesias J, Leyva A . A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes Dev. 2001; 15(16):2122-33. PMC: 312755. DOI: 10.1101/gad.204401. View

Kawasaki S, Borchert C, Deyholos M, Wang H, Brazille S, Kawai K . Gene expression profiles during the initial phase of salt stress in rice. Plant Cell. 2001; 13(4):889-905. PMC: 135538. DOI: 10.1105/tpc.13.4.889. View

Raghothama K . Phosphate transport and signaling. Curr Opin Plant Biol. 2000; 3(3):182-7. View

Deng X, Matsui M, Wei N, Wagner D, Chu A, Feldmann K . COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a G beta homologous domain. Cell. 1992; 71(5):791-801. DOI: 10.1016/0092-8674(92)90555-q. View

Del Pozo J, Allona I, Rubio V, Leyva A, de la Pena A, Aragoncillo C . A type 5 acid phosphatase gene from Arabidopsis thaliana is induced by phosphate starvation and by some other types of phosphate mobilising/oxidative stress conditions. Plant J. 1999; 19(5):579-89. DOI: 10.1046/j.1365-313x.1999.00562.x. View

Harmer S, Hogenesch J, Straume M, Chang H, Han B, Zhu T . Orchestrated transcription of key pathways in Arabidopsis by the circadian clock. Science. 2000; 290(5499):2110-3. DOI: 10.1126/science.290.5499.2110. View

Eisen M, Spellman P, Brown P, Botstein D . Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A. 1998; 95(25):14863-8. PMC: 24541. DOI: 10.1073/pnas.95.25.14863. View

TORRIANI A . From cell membrane to nucleotides: the phosphate regulon in Escherichia coli. Bioessays. 1990; 12(8):371-6. DOI: 10.1002/bies.950120804. View

Ogawa N, Derisi J, Brown P . New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis. Mol Biol Cell. 2000; 11(12):4309-21. PMC: 15074. DOI: 10.1091/mbc.11.12.4309. View

10.

Tepperman J, Zhu T, Chang H, Wang X, Quail P . Multiple transcription-factor genes are early targets of phytochrome A signaling. Proc Natl Acad Sci U S A. 2001; 98(16):9437-42. PMC: 55439. DOI: 10.1073/pnas.161300998. View

11.

Oh S, Lee S, Chung I, Lee C, Nam H . A senescence-associated gene of Arabidopsis thaliana is distinctively regulated during natural and artificially induced leaf senescence. Plant Mol Biol. 1996; 30(4):739-54. DOI: 10.1007/BF00019008. View

12.

Chen D, Delatorre C, Bakker A, Abel S . Conditional identification of phosphate-starvation-response mutants in Arabidopsis thaliana. Planta. 2000; 211(1):13-22. DOI: 10.1007/s004250000271. View

13.

Ma L, Zhao H, Deng X . Analysis of the mutational effects of the COP/DET/FUS loci on genome expression profiles reveals their overlapping yet not identical roles in regulating Arabidopsis seedling development. Development. 2003; 130(5):969-81. DOI: 10.1242/dev.00281. View

14.

Berben G, LEGRAIN M, Gilliquet V, Hilger F . The yeast regulatory gene PHO4 encodes a helix-loop-helix motif. Yeast. 1990; 6(5):451-4. DOI: 10.1002/yea.320060510. View

15.

Ma L, Gao Y, Qu L, Chen Z, Li J, Zhao H . Genomic evidence for COP1 as a repressor of light-regulated gene expression and development in Arabidopsis. Plant Cell. 2002; 14(10):2383-98. PMC: 151224. DOI: 10.1105/tpc.004416. View

16.

Burleigh S, Harrison M . The down-regulation of Mt4-like genes by phosphate fertilization occurs systemically and involves phosphate translocation to the shoots. Plant Physiol. 1999; 119(1):241-8. PMC: 32226. DOI: 10.1104/pp.119.1.241. View

17.

Theodorou M, Plaxton W . Metabolic Adaptations of Plant Respiration to Nutritional Phosphate Deprivation. Plant Physiol. 1993; 101(2):339-344. PMC: 160576. DOI: 10.1104/pp.101.2.339. View

18.

Trull M, Deikman J . An Arabidopsis mutant missing one acid phosphatase isoform. Planta. 1998; 206(4):544-50. DOI: 10.1007/s004250050431. View

19.

Helariutta Y, Fukaki H, Nakajima K, Jung J, Sena G, Hauser M . The SHORT-ROOT gene controls radial patterning of the Arabidopsis root through radial signaling. Cell. 2000; 101(5):555-67. DOI: 10.1016/s0092-8674(00)80865-x. View

20.

Abel S, Nurnberger T, Ahnert V, Krauss G, Glund K . Induction of an extracellular cyclic nucleotide phosphodiesterase as an accessory ribonucleolytic activity during phosphate starvation of cultured tomato cells. Plant Physiol. 2000; 122(2):543-52. PMC: 58891. DOI: 10.1104/pp.122.2.543. View