A Role for VASP in RhoA-Diaphanous Signalling to Actin Dynamics and SRF Activity

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2003 Jun 14

PMID 12805219

Citations 53

Authors

Robert Grosse

John W Copeland

Timothy P Newsome

Michael Way

Richard Treisman

Affiliations

Soon will be listed here.

Abstract

Vasodilator-stimulated phosphoprotein (VASP) is involved in multiple actin-mediated processes, including regulation of serum response factor (SRF) activity. We used the SRF transcriptional assay to define functional domains in VASP and to show that they coincide with those required for F-actin accumulation, as determined by a quantitative FACS assay. We identified inactive VASP mutants that can interfere both with F-actin assembly and with SRF activation by wild-type VASP. These VASP mutants also inhibit actin-based motility of Vaccinia virus and Shigella flexneri. VASP-induced F-actin accumulation and SRF activation require both functional Rho and its effector mDia, and conversely, mDia-mediated SRF activation is critically dependent on functional VASP. VASP and mDia also associate physically in vivo. These findings show that VASP and mDia function cooperatively downstream of Rho to control F-actin assembly and SRF activity.

Citing Articles

Genome maintenance meets mechanobiology.

Spegg V, Altmeyer M Chromosoma. 2023; 133(1):15-36.

PMID: 37581649 PMC: 10904543. DOI: 10.1007/s00412-023-00807-5.

Ena/VASP proteins at the crossroads of actin nucleation pathways in dendritic cell migration.

Visweshwaran S, Nayab H, Hoffmann L, Gil M, Liu F, Kuhne R Front Cell Dev Biol. 2022; 10:1008898.

PMID: 36274843 PMC: 9581539. DOI: 10.3389/fcell.2022.1008898.

Expression of the phagocytic receptors αβ and αβ is controlled by RIAM, VASP and Vinculin in neutrophil-differentiated HL-60 cells.

Torres-Gomez A, Fiyouzi T, Guerra-Espinosa C, Cardenes B, Clares I, Toribio V Front Immunol. 2022; 13:951280.

PMID: 36238292 PMC: 9552961. DOI: 10.3389/fimmu.2022.951280.

Myofibroblast dedifferentiation proceeds via distinct transcriptomic and phenotypic transitions.

Fortier S, Penke L, King D, Pham T, Ligresti G, Peters-Golden M JCI Insight. 2021; 6(6).

PMID: 33561015 PMC: 8026183. DOI: 10.1172/jci.insight.144799.

BCL11B Regulates Arterial Stiffness and Related Target Organ Damage.

Valisno J, May J, Singh K, Helm E, Venegas L, Budbazar E Circ Res. 2021; 128(6):755-768.

PMID: 33530702 PMC: 7969164. DOI: 10.1161/CIRCRESAHA.120.316666.

References

Smith G, Theriot J, Portnoy D . The tandem repeat domain in the Listeria monocytogenes ActA protein controls the rate of actin-based motility, the percentage of moving bacteria, and the localization of vasodilator-stimulated phosphoprotein and profilin. J Cell Biol. 1996; 135(3):647-60. PMC: 2121076. DOI: 10.1083/jcb.135.3.647. View

Gertler F, Niebuhr K, Reinhard M, Wehland J, Soriano P . Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics. Cell. 1996; 87(2):227-39. DOI: 10.1016/s0092-8674(00)81341-0. View

Laine R, Zeile W, Kang F, Purich D, Southwick F . Vinculin proteolysis unmasks an ActA homolog for actin-based Shigella motility. J Cell Biol. 1997; 138(6):1255-64. PMC: 2132544. DOI: 10.1083/jcb.138.6.1255. View

Niebuhr K, Ebel F, Frank R, Reinhard M, Domann E, Carl U . A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family. EMBO J. 1997; 16(17):5433-44. PMC: 1170174. DOI: 10.1093/emboj/16.17.5433. View

Dennler S, Itoh S, Vivien D, Ten Dijke P, Huet S, Gauthier J . Direct binding of Smad3 and Smad4 to critical TGF beta-inducible elements in the promoter of human plasminogen activator inhibitor-type 1 gene. EMBO J. 1998; 17(11):3091-100. PMC: 1170648. DOI: 10.1093/emboj/17.11.3091. View

Arsenian S, Weinhold B, Oelgeschlager M, Ruther U, Nordheim A . Serum response factor is essential for mesoderm formation during mouse embryogenesis. EMBO J. 1998; 17(21):6289-99. PMC: 1170954. DOI: 10.1093/emboj/17.21.6289. View

Zeile W, Condit R, LEWIS J, Purich D, Southwick F . Vaccinia locomotion in host cells: evidence for the universal involvement of actin-based motility sequences ABM-1 and ABM-2. Proc Natl Acad Sci U S A. 1998; 95(23):13917-22. PMC: 24964. DOI: 10.1073/pnas.95.23.13917. View

Frischknecht F, Cudmore S, Moreau V, Reckmann I, Rottger S, Way M . Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella. Curr Biol. 1999; 9(2):89-92. DOI: 10.1016/s0960-9822(99)80020-7. View

Rottner K, Behrendt B, Small J, Wehland J . VASP dynamics during lamellipodia protrusion. Nat Cell Biol. 1999; 1(5):321-2. DOI: 10.1038/13040. View

10.

Watanabe N, Kato T, Fujita A, Ishizaki T, Narumiya S . Cooperation between mDia1 and ROCK in Rho-induced actin reorganization. Nat Cell Biol. 1999; 1(3):136-43. DOI: 10.1038/11056. View

11.

Vasioukhin V, Bauer C, Yin M, Fuchs E . Directed actin polymerization is the driving force for epithelial cell-cell adhesion. Cell. 2000; 100(2):209-19. DOI: 10.1016/s0092-8674(00)81559-7. View

12.

Tominaga T, Sahai E, Chardin P, McCormick F, Courtneidge S, Alberts A . Diaphanous-related formins bridge Rho GTPase and Src tyrosine kinase signaling. Mol Cell. 2000; 5(1):13-25. DOI: 10.1016/s1097-2765(00)80399-8. View

13.

Grosse R, Roelle S, HERRLICH A, Hohn J, Gudermann T . Epidermal growth factor receptor tyrosine kinase mediates Ras activation by gonadotropin-releasing hormone. J Biol Chem. 2000; 275(16):12251-60. DOI: 10.1074/jbc.275.16.12251. View

14.

BEAR J, Loureiro J, LIBOVA I, Fassler R, Wehland J, Gertler F . Negative regulation of fibroblast motility by Ena/VASP proteins. Cell. 2000; 101(7):717-28. DOI: 10.1016/s0092-8674(00)80884-3. View

15.

Price C, Brindle N . Vasodilator-stimulated phosphoprotein is involved in stress-fiber and membrane ruffle formation in endothelial cells. Arterioscler Thromb Vasc Biol. 2000; 20(9):2051-6. DOI: 10.1161/01.atv.20.9.2051. View

16.

Harbeck B, Huttelmaier S, Schluter K, Jockusch B, Illenberger S . Phosphorylation of the vasodilator-stimulated phosphoprotein regulates its interaction with actin. J Biol Chem. 2000; 275(40):30817-25. DOI: 10.1074/jbc.M005066200. View

17.

Lambrechts A, Kwiatkowski A, Lanier L, BEAR J, Vandekerckhove J, Ampe C . cAMP-dependent protein kinase phosphorylation of EVL, a Mena/VASP relative, regulates its interaction with actin and SH3 domains. J Biol Chem. 2000; 275(46):36143-51. DOI: 10.1074/jbc.M006274200. View

18.

Frischknecht F, Way M . Surfing pathogens and the lessons learned for actin polymerization. Trends Cell Biol. 2001; 11(1):30-38. DOI: 10.1016/s0962-8924(00)01871-7. View

19.

BEAR J, Krause M, Gertler F . Regulating cellular actin assembly. Curr Opin Cell Biol. 2001; 13(2):158-66. DOI: 10.1016/s0955-0674(00)00193-9. View

20.

Reinhard M, Jarchau T, Walter U . Actin-based motility: stop and go with Ena/VASP proteins. Trends Biochem Sci. 2001; 26(4):243-9. DOI: 10.1016/s0968-0004(00)01785-0. View