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Utility of an In-house Mycobacteriophage-based Assay for Rapid Detection of Rifampin Resistance in Mycobacterium Tuberculosis Clinical Isolates

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Specialty Microbiology
Date 2003 Jun 7
PMID 12791894
Citations 13
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Abstract

A rapid in-house mycobacteriophage-based assay to identify multidrug resistance by detecting the rifampin susceptibility of Mycobacterium tuberculosis in a microtiter plate format was evaluated. The sensitivity, specificity, and overall accuracy of the assay were 100%. This test is rapid to perform and suitable for widespread implementation.

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References
1.
Espinal M, Laszlo A, Simonsen L, Boulahbal F, Kim S, Reniero A . Global trends in resistance to antituberculosis drugs. World Health Organization-International Union against Tuberculosis and Lung Disease Working Group on Anti-Tuberculosis Drug Resistance Surveillance. N Engl J Med. 2001; 344(17):1294-303. DOI: 10.1056/NEJM200104263441706. View

2.
Soini H, Musser J . Molecular diagnosis of mycobacteria. Clin Chem. 2001; 47(5):809-14. View

3.
Banaiee N, Bobadilla-Del-Valle M, Bardarov Jr S, Riska P, Small P, Ponce-de-Leon A . Luciferase reporter mycobacteriophages for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico. J Clin Microbiol. 2001; 39(11):3883-8. PMC: 88459. DOI: 10.1128/JCM.39.11.3883-3888.2001. View

4.
Somoskovi A, Parsons L, Salfinger M . The molecular basis of resistance to isoniazid, rifampin, and pyrazinamide in Mycobacterium tuberculosis. Respir Res. 2001; 2(3):164-8. PMC: 2002067. DOI: 10.1186/rr54. View

5.
Caws M, Drobniewski F . Molecular techniques in the diagnosis of Mycobacterium tuberculosis and the detection of drug resistance. Ann N Y Acad Sci. 2002; 953:138-45. DOI: 10.1111/j.1749-6632.2001.tb11371.x. View