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Activation of Caspase 9 in a Rat Model of Experimental Glaucoma

Overview
Journal Curr Eye Res
Publisher Informa Healthcare
Specialty Ophthalmology
Date 2003 Jun 6
PMID 12789547
Citations 28
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Abstract

Purpose: We investigated retinal ganglion cell (RGC) death and activation of caspase 9 in rats with experimental glaucoma.

Methods: Elevated intraocular pressure (IOP) was induced in rats using the Morrison model. Surviving backlabeled RGC were counted and TUNEL staining detected apoptosis. Procaspase 9 expression and activated caspase 9 were studied by immunoblot and immunohistochemistry.

Results: IOP correlated with surviving RGC. TUNEL-positive RGC were observed in animals with elevated IOP. Procaspase 9 levels increased with IOP intensity. Cleaved caspase 9 was detected by immunoblot only in rats with peak IOP above 35 mm Hg for > or =6 days. Cleaved caspase 9 staining was seen only in the ganglion cell layer of retinas from rats with peak IOP > or =32 mm Hg.

Conclusions: RGC loss is correlated with IOP in experimental glaucoma. These results support activation of caspase 9, the intrinsic caspase cascade, in RGC death in experimental glaucoma.

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