Runx3 Knockouts and Stomach Cancer

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2003 May 31

PMID 12776174

Citations 21

Authors

Ditsa Levanon

Ori Brenner

Florian Otto

Yoram Groner

Affiliations

Soon will be listed here.

Abstract

Gene targeting often results in knockout mice that show several phenotypes, some of which may not directly relate to the intrinsic function of the disrupted gene. Hence, to study the biological function of genes using knockout mice, one must identify the defects that are directly due to the loss of the targeted gene. Runx3 is a transcription factor that regulates lineage-specific gene expression in developmental processes. Recently, two groups produced Runx3 knockout mice. Two comparable defects were identified in both knockout strains, one involved neurogenesis and the other thymopoiesis. In addition, a stomach defect pertaining to gastric cancer was observed in one of the mutant strains, but not in the other. Here, we assess the differences between the two Runx3 mutant strains and discuss further studies that could reconcile these discrepancies. This article highlights the difficulties of inferring gene function through the interpretation of knockout phenotypes.

Citing Articles

Methylation of Promoter 2 in the Whole Blood of Children with Ulcerative Colitis.

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Loss of expression rather than cytoplasmic mislocalization of RUNX3 predicts worse outcome in non-small cell lung cancer.

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Epigenetic inactivation of in colorectal cancer.

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The clinicopathological significance of RUNX3 hypermethylation and mRNA expression in human breast cancer, a meta-analysis.

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