Induction of the Differentiation of Lentoids from Primate Embryonic Stem Cells

Overview

Journal Invest Ophthalmol Vis Sci

Specialty Ophthalmology

Date 2003 May 27

PMID 12766074

Citations 21

Authors

Sotaro Ooto

Masatoshi Haruta

Yoshihito Honda

Hiroshi Kawasaki

Yoshiki Sasai

Masayo Takahashi

Affiliations

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Abstract

Purpose: To produce lens cells from primate embryonic stem (ES) cells in a reproducible, controlled manner.

Methods: Cynomologus monkey ES cells were induced to differentiate by stromal cell-derived inducing activity (SDIA). The lentoids produced by this treatment were processed for immunohistochemical and immunoblotting analysis. The effect of varying the concentration of fibroblast growth factor (FGF)-2 and the density of the ES colonies plated during the differentiation process were also examined.

Results: After a 2- to 3-week induction period, lentoids were produced by a subpopulation of ES colonies. Western blot analysis and immunohistochemistry revealed that these lentoids expressed alphaA-crystallin and Pax6. The number of lentoids resulting from treatment increased with increasing FGF-2 concentration and plated colony density.

Conclusions: The differentiation of primate ES cells into lentoids can be achieved by treatment with SIDA. ES cells can be used to facilitate a greater understanding of the mechanisms functioning in differentiation in vivo and in vitro.

Citing Articles

Lens Regeneration: The Application of iSyTE and In Silico Approaches to Evaluate Gene Expression in Lens Organoids.

Shrestha S, Lachke S Methods Mol Biol. 2024; 2848:37-58.

PMID: 39240515 DOI: 10.1007/978-1-0716-4087-6_3.

Generation of Functional Lentoid Bodies from Human-Induced Pluripotent Stem Cells.

Qin Z, Yao K, Fu Q Methods Mol Biol. 2024; 2848:25-36.

PMID: 39240514 DOI: 10.1007/978-1-0716-4087-6_2.

Lens regeneration using hESCs-derived cells -similar to natural lens.

Chen X, Wang H, Chen H, Ren L, Wang W, Xu J iScience. 2023; 26(6):106921.

PMID: 37378332 PMC: 10291466. DOI: 10.1016/j.isci.2023.106921.

Generation of Lens Progenitor Cells and Lentoid Bodies from Pluripotent Stem Cells: Novel Tools for Human Lens Development and Ocular Disease Etiology.

Cvekl A, Camerino M Cells. 2022; 11(21).

PMID: 36359912 PMC: 9658148. DOI: 10.3390/cells11213516.

RNA-binding proteins and post-transcriptional regulation in lens biology and cataract: Mediating spatiotemporal expression of key factors that control the cell cycle, transcription, cytoskeleton and transparency.

Lachke S Exp Eye Res. 2021; 214:108889.

PMID: 34906599 PMC: 8792301. DOI: 10.1016/j.exer.2021.108889.