The VirR/VirS Regulatory Cascade Affects Transcription of Plasmid-encoded Putative Virulence Genes in Clostridium Perfringens Strain 13

Overview

Journal FEMS Microbiol Lett

Publisher Oxford University Press

Specialty Microbiology

Date 2003 May 22

PMID 12757957

Citations 31

Authors

Kaori Ohtani

Hameem I Kawsar

Kayo Okumura

Hideo Hayashi

Tohru Shimizu

Affiliations

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Abstract

We analyzed the transcriptional regulation of the putative virulence genes encoded on the plasmid pCP13 from Clostridium perfringens strain 13. The transcription of the beta2-toxin (cpb2) and possible collagen adhesin (cna) genes were regulated in both a positive and negative manner, respectively, by the two-component VirR/VirS system. The secondary regulator of the VirR/VirS system, VR-RNA, also affects the expression of both of these genes in the same fashion as the VirR/VirS system. This indicates that the global regulatory cascade of the VirR/VirS system controls the expression of virulence genes located on the plasmid, as well as those found chromosomally in C. perfringens strain 13.

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