Metal Ion Enhancement of Fungal Growth, Gene Expression and Aflatoxin Synthesis in Aspergillus Flavus: RT-PCR Characterization

Overview

Journal J Appl Microbiol

Publisher Oxford University Press

Date 2003 May 20

PMID 12752802

Citations 20

Authors

R Cuero

T Ouellet

J Yu

N Mogongwa

Affiliations

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Abstract

Aims: To determine the effect of mineral ions (e.g., Zn2+, Cu2+, and Fe2+) on the enhancement of fungal growth, total RNA, aflatoxin pathway gene expression, and production of aflatoxin and its precursor O-methylsterigmatocystin (OMST).

Methods And Results: The influence of the metal ions, as a single or mixed treatments, was observed in submerged cultures of toxigenic Aspergillus flavus through changes in the fungal RNA or aflatoxin pathway gene (omtA) by reverse transcription-polymerase chain reaction (RT-PCR) levels, and also in fungal dry-weight accumulation, aflatoxin and OMST production.

Conclusions: The ion treatments induced changes of fungal total RNA, mRNA levels, associate fungal growth, biosynthesis of aflatoxin and OMST, and enhanced expression of RT-PCR.

Significance And Impact Of Study: Demonstrates at the cellular and molecular level, the significant effects of metal ions on both fungal growth and production of aflatoxin.

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