Human Bone Marrow Endothelial Cells Elaborate Non-stromal-cell-derived Factor-1 (SDF-1)-dependent Chemoattraction and SDF-1-dependent Transmigration of Haematopoietic Progenitors

Overview

Journal Br J Haematol

Specialty Hematology

Date 2003 May 20

PMID 12752108

Citations 7

Authors

Deog-Yeon Jo

Jin-Hee Hwang

Jin-Man Kim

Hwan-Jung Yun

Samyong Kim

Affiliations

Soon will be listed here.

Abstract

This study investigated human bone marrow endothelial cells (BMEC) chemoattractive activity in relation to haematopoietic cell trafficking. BMEC-conditioned medium induced chemoattraction of haematopoietic progenitor cells. Migration was not inhibited by pretreating the cells with pertussis toxin (PTX) or 12G5, indicating that the chemoattractive activity was not dependent on stromal-cell-derived factor-1 (SDF-1). Spontaneous migration, but not SDF-1-mediated chemotaxis of haematopoietic progenitors, was better supported by BMEC as compared with umbilical vein endothelial cells. The superior migration was abolished by pretreating the cells with PTX, indicating that BMEC-derived SDF-1 favours bone marrow endothelium, with better transmigration of haematopoietic progenitors.

Citing Articles

A biomimetic nanocomposite with enzyme-like activities and CXCR4 antagonism efficiently enhances the therapeutic efficacy of acute myeloid leukemia.

Kong F, He H, Bai H, Yang F, Ma M, Gu N Bioact Mater. 2022; 18:526-538.

PMID: 35415298 PMC: 8976099. DOI: 10.1016/j.bioactmat.2022.03.022.

Synovial inflammation plays a greater role in post-traumatic osteoarthritis compared to idiopathic osteoarthritis in the Hartley guinea pig knee.

Thomas N, Wu W, Fleming B, Wei F, Chen Q, Wei L BMC Musculoskelet Disord. 2017; 18(1):556.

PMID: 29284451 PMC: 5747041. DOI: 10.1186/s12891-017-1913-6.

Attenuation of cartilage pathogenesis in post-traumatic osteoarthritis (PTOA) in mice by blocking the stromal derived factor 1 receptor (CXCR4) with the specific inhibitor, AMD3100.

Thomas N, Li P, Fleming B, Chen Q, Wei X, Xiao-Hua P J Orthop Res. 2015; 33(7):1071-8.

PMID: 25732515 PMC: 4557642. DOI: 10.1002/jor.22862.

A designed peptide targeting CXCR4 displays anti-acute myelocytic leukemia activity in vitro and in vivo.

Li X, Guo H, Yang Y, Meng J, Liu J, Wang C Sci Rep. 2014; 4:6610.

PMID: 25312253 PMC: 4196105. DOI: 10.1038/srep06610.

Attenuation of osteoarthritis via blockade of the SDF-1/CXCR4 signaling pathway.

Wei F, Moore D, Wei L, Li Y, Zhang G, Wei X Arthritis Res Ther. 2012; 14(4):R177.

PMID: 22849584 PMC: 3580571. DOI: 10.1186/ar3930.