Is Automated Platelet Counting Still a Problem in Thrombocytopenic Blood?

Overview

Journal Sao Paulo Med J

Specialty General Medicine

Date 2003 May 20

PMID 12751339

Citations 2

Authors

Raimundo Antonio Gomes Oliveira

Maria Mariko Takadachi

Kimiyo Nonoyama

Orlando Cesar de Oliveira Barretto

Affiliations

Soon will be listed here.

Abstract

Context: Reliable platelet counting is crucial for indicating prophylactic platelet transfusion in thrombocytopenic patients.

Objective: To evaluate the precision and accuracy of platelet counting for thrombocytopenic patients, using four different automated counters in comparison with the Brecher & Cronkite reference method recommended by the International Committee for Standardization in Hematology (ICSH).

Type Of Study: Automated platelet counting assessment in thrombocytopenic patients.

Setting: Hematology Laboratory, Hospital do Servidor Público Estadual de São Paulo, and the Hematology Division of Instituto Adolfo Lutz, São Paulo, SP, Brazil.

Main Measurements: Brecher & Cronkite reference method and four different automated platelet counters.

Participants: 43 thrombocytopenic patients with platelet counts of less than 30,000/microliter.

Results: The ADVIA-120 (Bayer), Coulter STKS, H1 System (Technicom-Bayer) and Coulter T-890 automatic instruments presented great precision and accuracy in relation to laboratory thrombocytopenic samples obtained by diluting blood from normal donors. However, when thrombocytopenic patients were investigated, all the counters except ADVIA (which is based on volume and refraction index) showed low accuracy when compared to the Brecher & Cronkite reference method (ICSH). The ADVIA counter showed high correlation (r = 0.974). However, all counters showed flags in thrombocytopenic samples.

Conclusion: The Brecher & Cronkite reference method should always be indicated in thrombocytopenic patients for platelet counts below 30,000 plt/microliter obtained in one dimensional counters.

Citing Articles

Reproducibility of Manual Platelet Estimation Following Automated Low Platelet Counts.

Al-Hosni Z, Al-Khabori M, Al-Mamari S, Al-Qasabi J, Davis H, Al-Lawati H Oman Med J. 2016; 31(6):409-413.

PMID: 27974955 PMC: 5099402. DOI: 10.5001/omj.2016.83.

Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications.

Lambert M, Rauova L, Bailey M, Sola-Visner M, Kowalska M, Poncz M Blood. 2007; 110(4):1153-60.

PMID: 17495129 PMC: 1976471. DOI: 10.1182/blood-2007-01-067116.

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