Detection of RNA Viruses in Sudden Infant Death (SID)

Overview

Journal Int J Legal Med

Specialty Forensic Sciences

Date 2003 May 17

PMID 12750907

Citations 15

Authors

T Bajanowski

B Rolf

G Jorch

B Brinkmann

Affiliations

Soon will be listed here.

Abstract

Continuing previous work, reverse transcriptase polymerase chain reaction approaches for the detection of RNA viruses (influenza A+B, parainfluenza virus 3, respiratory syncytial virus) were developed. A total of 118 infant deaths, including 13 cases of non-natural death (nND), 78 cases of natural death (ND) without morphological signs of interstitial pneumonia (IP), 27 cases showing IP (ND+IP), were investigated using frozen lung tissue ( N=100) and paraffin-embedded material ( N=18). In five of the autopsy cases (ND+IP) the influenza B virus genome could be detected and the other types of viruses were completely negative. Together with previous results (detection of adenoviruses and cytomegaloviruses) in the same groups, the frequency of virus detection in the cases with IP was 48% compared with 14% in the ND without IP and 7% in the nND. Significant differences in the frequency of virus detection were also obtained when the cases were divided in SIDS and non-SIDS. The results obtained indicate an association between IP and some viruses and support the hypothesis that respiratory virus infections could act as trigger in sudden infant death.

Citing Articles

The vicious spiral in Sudden Infant Death Syndrome.

Opdal S, Stray-Pedersen A, Eidahl J, Vege A, Ferrante L, Rognum T Front Pediatr. 2025; 13:1487000.

PMID: 40013115 PMC: 11862695. DOI: 10.3389/fped.2025.1487000.

Metabolic acidosis and sudden infant death syndrome: overlooked data provides insight into SIDS pathogenesis.

Goldwater P, Gebien D World J Pediatr. 2024; 21(1):29-40.

PMID: 39656413 PMC: 11814015. DOI: 10.1007/s12519-024-00860-9.

Sudden Infant Death Syndrome (SIDS): State of the Art and Future Directions.

Fraile-Martinez O, Garcia-Montero C, Diez S, Bravo C, Quintana-Coronado M, Lopez-Gonzalez L Int J Med Sci. 2024; 21(5):848-861.

PMID: 38617004 PMC: 11008475. DOI: 10.7150/ijms.89490.

Current SIDS research: time to resolve conflicting research hypotheses and collaborate.

Goldwater P Pediatr Res. 2023; 94(4):1273-1277.

PMID: 37173404 PMC: 10175898. DOI: 10.1038/s41390-023-02611-4.

Pulmonary immune profiling of SIDS: impaired immune maturation and age-related cytokine imbalance.

Qu D, Engelmann T, Preuss V, Hagemeier L, Radomsky L, Beushausen K Pediatr Res. 2022; 93(5):1239-1249.

PMID: 35986144 PMC: 10132963. DOI: 10.1038/s41390-022-02203-8.

References

Blackwell C, Saadi A, Raza M, WEIR D, Busuttil A . The potential role of bacterial toxins in sudden infant death syndrome (SIDS). Int J Legal Med. 1993; 105(6):333-8. DOI: 10.1007/BF01222118. View

Karron R, Froehlich J, Bobo L, Belshe R, Yolken R . Rapid detection of parainfluenza virus type 3 RNA in respiratory specimens: use of reverse transcription-PCR-enzyme immunoassay. J Clin Microbiol. 1994; 32(2):484-8. PMC: 263059. DOI: 10.1128/jcm.32.2.484-488.1994. View

Budowle B, Chakraborty R, Giusti A, Eisenberg A, Allen R . Analysis of the VNTR locus D1S80 by the PCR followed by high-resolution PAGE. Am J Hum Genet. 1991; 48(1):137-44. PMC: 1682756. View

Nishimura T, Fujiyoshi Y, Irie K, Tanimura A . Electron microscopic observations of influenzal pneumonitis. Kurume Med J. 1985; 32(4):263-71. DOI: 10.2739/kurumemedj.32.263. View

Allen R, GRAVES G, Budowle B . Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver. Biotechniques. 1989; 7(7):736-44. View

Shimizu C, Rambaud C, Cheron G, Rouzioux C, Lozinski G, Rao A . Molecular identification of viruses in sudden infant death associated with myocarditis and pericarditis. Pediatr Infect Dis J. 1995; 14(7):584-8. DOI: 10.1097/00006454-199507000-00006. View

Moller A, Meyer E, Brinkmann B . Different types of structural variation in STRs: HumFES/FPS, HumVWA and HumD21S11. Int J Legal Med. 1994; 106(6):319-23. DOI: 10.1007/BF01224779. View

Schaefer H . Inflammatory disease of the human lung of definite or presumed viral origin. Cytologic and histologic topics. Curr Top Pathol. 1983; 73:153-205. DOI: 10.1007/978-3-642-69134-8_4. View

STOLTENBERG L, Saugstad O, Rognum T . Sudden infant death syndrome victims show local immunoglobulin M response in tracheal wall and immunoglobulin A response in duodenal mucosa. Pediatr Res. 1992; 31(4 Pt 1):372-5. DOI: 10.1203/00006450-199204000-00013. View

10.

Grangeot-Keros L, Broyer M, Briand E, Gut J, Turkoglu S, Chretien P . Enterovirus in sudden unexpected deaths in infants. Pediatr Infect Dis J. 1996; 15(2):123-8. DOI: 10.1097/00006454-199602000-00007. View

11.

An S, Gould S, Keeling J, Fleming K . Role of respiratory viral infection in SIDS: detection of viral nucleic acid by in situ hybridization. J Pathol. 1993; 171(4):271-8. DOI: 10.1002/path.1711710407. View

12.

Yolken R, Murphy M . Sudden infant death syndrome associated with rotavirus infection. J Med Virol. 1982; 10(4):291-6. DOI: 10.1002/jmv.1890100409. View

13.

Bajanowski T, Wiegand P, Cecchi R, Pring-Akerblom P, Adrian T, Jorch G . Detection and significance of adenoviruses in cases of sudden infant death. Virchows Arch. 1996; 428(2):113-8. DOI: 10.1007/BF00193939. View

14.

Dettmeyer R, Kandolf R, Schmidt P, Schlamann M, Madea B . Lympho-monocytic enteroviral myocarditis: traditional, immunohistological and molecularpathological methods for diagnosis in a case of suspected sudden infant death syndrome (SIDS). Forensic Sci Int. 2001; 119(1):141-4. DOI: 10.1016/s0379-0738(00)00413-8. View

15.

Zhang W, Evans D . Detection and identification of human influenza viruses by the polymerase chain reaction. J Virol Methods. 1991; 33(1-2):165-89. DOI: 10.1016/0166-0934(91)90017-t. View

16.

Bajanowski T, Furst P, Wilmers K, Beike J, Kohler H, Brinkmann B . Dioxin in infants--an environmental hazard?. Int J Legal Med. 2002; 116(1):27-32. DOI: 10.1007/s004140100257. View

17.

Coumbe A, Fox J, Briggs M, Tedder R, Berry C . Cytomegalovirus and human herpesvirus-6 in sudden infant death syndrome: an in situ hybridization study. Pediatr Pathol. 1990; 10(4):483-90. DOI: 10.3109/15513819009067137. View

18.

Cecchi R, Bajanowski T, Kahl B, Wiegand P . CMV-DNA detection in parenchymatous organs in cases of SIDS. Int J Legal Med. 1995; 107(6):291-5. DOI: 10.1007/BF01246875. View