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Comparison of Lateral-flow Immunoassay and Enzyme Immunoassay with Viral Culture for Rapid Detection of Influenza Virus in Nasal Wash Specimens from Children

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2003 May 8
PMID 12734259
Citations 18
Authors
Andreea C Cazacu
Jewel Greer
Moe Taherivand
Gail J Demmler
Affiliations
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Abstract

The performance of two commercially available rapid test kits for influenza virus detection was compared to that of viral culture by using 356 nasal wash specimens collected during the 2001 to 2002 influenza season. Overall, the two rapid tests were easy to perform and showed comparable sensitivities (70.4 and 72.2%) and specificities (97.7 and 98.3%); for both test kit groups, most of the specimens that yielded false-negative results were found to be growing influenza B virus.

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References
1.
Poehling K, Griffin M, Dittus R, Tang Y, Holland K, Li H . Bedside diagnosis of influenzavirus infections in hospitalized children. Pediatrics. 2002; 110(1 Pt 1):83-8. DOI: 10.1542/peds.110.1.83. View
2.
Demmler G . Laboratory diagnosis of influenza: recent advances. Semin Pediatr Infect Dis. 2002; 13(2):85-9. DOI: 10.1053/spid.2002.122994. View
3.
Troendle J, Demmler G, Glezen W, Finegold M, Romano M . Fatal influenza B virus pneumonia in pediatric patients. Pediatr Infect Dis J. 1992; 11(2):117-21. DOI: 10.1097/00006454-199202000-00011. View
4.
Noyola D, Clark B, ODONNELL F, Atmar R, Greer J, Demmler G . Comparison of a new neuraminidase detection assay with an enzyme immunoassay, immunofluorescence, and culture for rapid detection of influenza A and B viruses in nasal wash specimens. J Clin Microbiol. 2000; 38(3):1161-5. PMC: 86363. DOI: 10.1128/JCM.38.3.1161-1165.2000. View
5.
Steininger C, Kundi M, Aberle S, Aberle J, Popow-Kraupp T . Effectiveness of reverse transcription-PCR, virus isolation, and enzyme-linked immunosorbent assay for diagnosis of influenza A virus infection in different age groups. J Clin Microbiol. 2002; 40(6):2051-6. PMC: 130702. DOI: 10.1128/JCM.40.6.2051-2056.2002. View