In Vitro and in Ovo Expression of Chicken Gamma Interferon by a Defective RNA of Avian Coronavirus Infectious Bronchitis Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2003 Apr 30

PMID 12719562

Citations 12

Authors

Karen Hackney

Dave Cavanagh

Pete Kaiser

Paul Britton

Affiliations

Soon will be listed here.

Abstract

Coronavirus defective RNAs (D-RNAs) have been used for site-directed mutagenesis of coronavirus genomes and for expression of heterologous genes. D-RNA CD-61 derived from the avian coronavirus infectious bronchitis virus (IBV) was used as an RNA vector for the expression of chicken gamma interferon (chIFN-gamma). D-RNAs expressing chIFN-gamma were shown to be capable of rescue, replication, and packaging into virions in a helper virus-dependent system following electroporation of in vitro-derived T7 RNA transcripts into IBV-infected cells. Secreted chIFN-gamma, under the control of an IBV transcription-associated sequence derived from gene 5 of the Beaudette strain, was expressed from two different positions within CD-61 and shown to be biologically active. In addition, following infection of 10-day-old chicken embryos with IBV containing D-RNAs expressing chIFN-gamma, the allantoic fluid was shown to contain biologically active chIFN-gamma, demonstrating that IBV D-RNAs can express heterologous genes in vivo.

Citing Articles

Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016.

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CpG-ODNs induced changes in cytokine/chemokines genes expression associated with suppression of infectious bronchitis virus replication in chicken lungs.

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Infectious Bronchitis Virus as a Vector for the Expression of Heterologous Genes.

Bentley K, Armesto M, Britton P PLoS One. 2013; 8(6):e67875.

PMID: 23840781 PMC: 3694013. DOI: 10.1371/journal.pone.0067875.

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

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