Antibiotic-resistant Mutants of Bacillus Subtilis Conditional for Sporulation

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1975 Jan 1

PMID 127112

Citations 14

Authors

R S Graham

K F Bott

Affiliations

Soon will be listed here.

Abstract

Among spontaneously occurring antibiotic-resistant mutants of Bacillus subtilis 168 we have identified a sub-class that is conditionally sporulative. Mutants in this sub-class are resistant to antibiotic during vegetative growth but are sensitive during sporulation. Mutants conditionally-resistant to erythromycin, kanamycin, spectinomycin, and streptomycin have been isolated and characterized by phase contrast microscopy and with respect to their ability to synthesize heat-resistant endospores or the sporulation-associated enzyme alkaline phosphatase. The results suggest that several entirely different genetic lesions may result in this single phenotype. This group includes mutants whose properties suggest that both th 30S and 50S ribosomal subunits may be altered concomitant with early spore specific metabolism. The blockage imposed by antibiotic may be at or near Stage 2 of sporulation.

Citing Articles

The use of antibiotics for studies of morphogenesis and differentiation in microorganisms.

Betina V Folia Microbiol (Praha). 1980; 25(6):505-23.

PMID: 7002750 DOI: 10.1007/BF02897219.

Revertants of a streptomycin-resistant, oligosporogenous mutant of Bacillus subtilis.

Henkin T, Campbell K, Chambliss G Mol Gen Genet. 1982; 186(3):347-54.

PMID: 6811835 DOI: 10.1007/BF00729453.

Inhibitory action of erythromycin on bacteriophage SPO1 multiplication in sporulating cells of Bacillus subtilis 168.

Hirochika H Mol Gen Genet. 1980; 179(3):581-8.

PMID: 6777627 DOI: 10.1007/BF00271748.

Mutation affecting expression of spectinomycin resistance in Bacillus subtilis.

Cannon J, Bott K J Bacteriol. 1980; 141(1):409-12.

PMID: 6444408 PMC: 293614. DOI: 10.1128/jb.141.1.409-412.1980.

Streptomycin-resistant, asporogenous mutant of Bacillus subtilis.

Campbell K, Chambliss G Mol Gen Genet. 1977; 158(2):193-200.

PMID: 414073 DOI: 10.1007/BF00268313.

References

Tanaka K, Tamaki M, Osawa S, Kimura A, Takata R . Erythromycin resistant mutants of Bacillus subtilis. Mol Gen Genet. 1973; 127(2):157-61. DOI: 10.1007/BF00333663. View

Weisblum B, Davies J . Antibiotic inhibitors of the bacterial ribosome. Bacteriol Rev. 1968; 32(4 Pt 2):493-528. PMC: 413162. View

Vold B . Analysis of isoaccepting transfer ribonucleic acid species of Bacillus subtilis: changes in chromatography of transfer ribonucleic acids associated with stage of development. J Bacteriol. 1973; 114(1):178-82. PMC: 251754. DOI: 10.1128/jb.114.1.178-182.1973. View

Kaneko I, Doi R . Alteration of valyl-sRNA during sporulation of bacillus subtilis. Proc Natl Acad Sci U S A. 1966; 55(3):564-71. PMC: 224188. DOI: 10.1073/pnas.55.3.564. View

Cundliffe E . Intracellular distribution of ribosomes and polyribosomes in Bacillus megaterium. J Mol Biol. 1970; 52(3):467-81. DOI: 10.1016/0022-2836(70)90413-4. View

Harford N, Sueoka N . Chromosomal location of antibiotic resistance markers in Bacillus subtilis. J Mol Biol. 1970; 51(2):267-86. DOI: 10.1016/0022-2836(70)90142-7. View

Fortnagel P, Bergmann R . Alteration of the ribosomal fraction of Bacillus subtilis during sporulation. Biochim Biophys Acta. 1973; 299(1):136-41. DOI: 10.1016/0005-2787(73)90404-8. View

Chuang R, Doi R . Characterization of lysine transfer ribonucleic acid from vegetative cells and spores of Bacillus subtilis. J Biol Chem. 1972; 247(11):3476-84. View

Smith I, Goldthwaite C, Dubnau D . The genetics of ribosomes in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1969; 34:85-9. DOI: 10.1101/sqb.1969.034.01.013. View

10.

Vold B . Variations in activity of aminoacyl-tRNA synthetases as a function of development in Bacillus subtilis. Arch Biochem Biophys. 1973; 154(2):691-5. DOI: 10.1016/0003-9861(73)90024-6. View

11.

Goldthwaite C, Smith I . Physiological characterization of antibiotic resistant mutants of Bacillus subtilis. Mol Gen Genet. 1972; 114(3):190-204. DOI: 10.1007/BF01788888. View

12.

STERLINI J, MANDELSTAM J . Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969; 113(1):29-37. PMC: 1184601. DOI: 10.1042/bj1130029. View

13.

Osawa S, Takata R, Tanaka K, Tamaki M . Chloramphenicol resistant mutants of Bacillus subtilis. Mol Gen Genet. 1973; 127(2):163-73. DOI: 10.1007/BF00333664. View

14.

Kobayashi Y, HALVORSON H . Evidence for a defective protein synthesizing system in dormant spores of Bacillus cereus. Arch Biochem Biophys. 1968; 123(3):622-32. DOI: 10.1016/0003-9861(68)90182-3. View

15.

Kimura A, Kobata K, Takata R, Osawa S . Genetic and chemical studies of ribosomes from spectinomycin resistant mutants of Bacillus subtilis. Mol Gen Genet. 1973; 124(2):107-15. DOI: 10.1007/BF00265144. View

16.

Bott K . Altered sporulation and respiratory patterns in mutants of Bacillus subtilis induced by acridine orange. J Bacteriol. 1966; 92(1):229-40. PMC: 276220. DOI: 10.1128/jb.92.1.229-240.1966. View

17.

Milhaud P, Balassa G . Biochemical genetics of bacterial sporulation. IV. Sequential development of resistances to chemical and physical agents during sporulation of Bacillus subtilis. Mol Gen Genet. 1973; 125(3):241-50. DOI: 10.1007/BF00270746. View

18.

Staal S, Hoch J . Conditional dihydrostreptomycin resistance in Bacillus subtilis. J Bacteriol. 1972; 110(1):202-7. PMC: 247399. DOI: 10.1128/jb.110.1.202-207.1972. View

19.

Losick R . In vitro transcription. Annu Rev Biochem. 1972; 41:409-46. DOI: 10.1146/annurev.bi.41.070172.002205. View

20.

BERGERE J, HERMIER J . [STUDY OF FACTORS INVOLVED IN THE MASSIVE PRODUCTION OF BACILLUS SUBTILIS SPORES]. Ann Inst Pasteur (Paris). 1964; 106:214-35. View