First in Vitro Directed Biosynthesis of New Compounds by a Minimal Type II Polyketide Synthase: Evidence for the Mechanism of Chain Length Determination

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2003 Apr 22

PMID 12703773

Citations 12

Authors

T P Nicholson

C Winfield

J Westcott

J Crosby

T J Simpson

R J Cox

Affiliations

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Abstract

The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.

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