Streptococcus Mutans Dextransucrase: Availability of Disaggregated Enzyme After Growth in a Chemically Defined Medium

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1976 May 1

PMID 1270153

Citations 16

Authors

C F Schachtele

S K Harlander

G R Germaine

Affiliations

Soon will be listed here.

Abstract

The soluble dextransucrase (EC 2.4.1.5) activity produced by Streptococcus mutans strain 6715 during growth on a chemically defined synthetic medium (FMS) was compared to enzyme from glucose broth cultures (TSB). Growth on the two media was similar. The specific activity of ammonium sulfate-precipitated FMC enzyme was 17 times greater than similar TSB enzyme preparations. The FMC enzyme was stimulated 11-fold, whereas the TSB enzyme was stimulated 1.2-fold by the addition of exogenous primer dextran. In contrast to the TSB enzyme, the FMC activity could be disaggregated to a low-molecular-weight form by 1 M salt. Thus, low-molecular-weight S. mutans dextransucrase activity free of contaminating primer glucan may be readily obtained after growth of the bacterium in a chemically defined sucrose-free medium.

Citing Articles

Biology, immunology, and cariogenicity of Streptococcus mutans.

Hamada S, Slade H Microbiol Rev. 1980; 44(2):331-84.

PMID: 6446023 PMC: 373181. DOI: 10.1128/mr.44.2.331-384.1980.

Expression of a Streptococcus mutans glucosyltransferase gene in Escherichia coli.

Robeson J, Barletta R, Curtiss 3rd R J Bacteriol. 1983; 153(1):211-21.

PMID: 6217191 PMC: 217359. DOI: 10.1128/jb.153.1.211-221.1983.

Effects of local immunization with glucosyltransferase on colonization of hamsters by Streptococcus mutans.

Smith D, Taubman M, Ebersole J Infect Immun. 1982; 37(2):656-61.

PMID: 6214510 PMC: 347582. DOI: 10.1128/iai.37.2.656-661.1982.

Purification and properties of glucosyltransferase responsible for water-insoluble glucan synthesis from Streptococcus mutans.

Fukui K, Moriyama T, Miyake Y, Mizutani K, Tanaka O Infect Immun. 1982; 37(1):1-9.

PMID: 6179873 PMC: 347481. DOI: 10.1128/iai.37.1.1-9.1982.

Streptococcus mutans dextransucrase: effect of cerulenin on lipid synthesis and enzyme production.

Leung W, Harlander S, Schachtele C Infect Immun. 1980; 28(3):846-52.

PMID: 6156908 PMC: 551028. DOI: 10.1128/iai.28.3.846-852.1980.

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