Identification and Characterization of Nodulation-signaling Pathway 2, a Gene of Medicago Truncatula Involved in Nod Actor Signaling

Overview

Journal Plant Physiol

Specialty Physiology

Date 2003 Mar 20

PMID 12644655

Citations 69

Authors

Giles E D Oldroyd

Sharon R Long

Affiliations

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Abstract

Bacterially derived Nod factor is critical in the establishment of the legume/rhizobia symbiosis. Understanding the mechanisms of Nod factor perception and signal transduction in the plant will greatly advance our understanding of this complex interaction. Here, we describe the identification of a new locus, nodulation-signaling pathway 2 (NSP2), of Medicago truncatula that is involved in Nod factor signaling. Mutants at this locus are blocked for Nod factor-induced gene expression and show a reduced root hair deformation response. nsp2 plants also show a complete absence of infection and cortical cell division following Sinorhizobium meliloti inoculation. Nod factor-induced calcium spiking, one of the earliest responses tested, is still functional in these mutant plants. We conclude that the gene NSP2 is a component of the Nod factor signal transduction pathway that lies downstream of the calcium-spiking response.

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