DTASelect and Contrast: Tools for Assembling and Comparing Protein Identifications from Shotgun Proteomics

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2003 Mar 20

PMID 12643522

Citations 759

Authors

David L Tabb

W Hayes McDonald

John R Yates 3rd

Affiliations

Soon will be listed here.

Abstract

The components of complex peptide mixtures can be separated by liquid chromatography, fragmented by tandem mass spectrometry, and identified by the SEQUEST algorithm. Inferring a mixture's source proteins requires that the identified peptides be reassociated. This process becomes more challenging as the number of peptides increases. DTASelect, a new software package, assembles SEQUEST identifications and highlights the most significant matches. The accompanying Contrast tool compares DTASelect results from multiple experiments. The two programs improve the speed and precision of proteomic data analysis.

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