Close Identity of a Pressure-stabilized Intermediate with a Kinetic Intermediate in Protein Folding

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2003 Mar 12

PMID 12629216

Citations 28

Authors

Ryo Kitahara

Kazuyuki Akasaka

Affiliations

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Abstract

Atomic detailed structural study of a transiently existing folding intermediate is severely limited because of its short life. In ubiquitin, we found that a pressure-stabilized equilibrium conformer shares a common structural feature with the proline-trapped kinetic intermediate found in a pulse-labeling (1)H(2)H exchange NMR study [Briggs, M. S. & Roder, H. (1992) Proc. Natl. Acad. Sci. USA 89, 2017-2021]. The conformer is locally unfolded in the entire segment from residues 33 to 42 and in C-terminal residues 70-76. The close structural identity of an equilibrium intermediate stabilized under pressure with a transiently observed folding intermediate is likely to be general in terms of a folding funnel common to both experiments.

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