Principal Role of TRAP/mediator and SWI/SNF Complexes in Kaposi's Sarcoma-associated Herpesvirus RTA-mediated Lytic Reactivation

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2003 Mar 4

PMID 12612078

Citations 74

Authors

Yousang Gwack

Hwa Jin Baek

Hiroyuki Nakamura

Sun Hwa Lee

Michael Meisterernst

Robert G Roeder

Jae U Jung

Affiliations

Soon will be listed here.

Abstract

An important step in the herpesvirus life cycle is the switch from latency to lytic reactivation. The RTA transcription activator of Kaposi's sarcoma-associated herpesvirus (KSHV) acts as a molecular switch for lytic reactivation. Here we demonstrate that KSHV RTA recruits CBP, the SWI/SNF chromatin remodeling complex, and the TRAP/Mediator coactivator into viral promoters through interactions with a short acidic sequence in the carboxyl region and that this recruitment is essential for RTA-dependent viral gene expression. The Brg1 subunit of SWI/SNF and the TRAP230 subunit of TRAP/Mediator were shown to interact directly with RTA. Consequently, genetic ablation of these interactions abolished KSHV lytic replication. These results demonstrate that the recruitment of CBP, SWI/SNF, and TRAP/Mediator complexes by RTA is the principal mechanism to direct well-controlled viral gene expression and thereby viral lytic reactivation.

Citing Articles

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