The Production of Antibody Fragments and Antibody Fusion Proteins by Yeasts and Filamentous Fungi

Overview

Journal Microb Cell Fact

Publisher Biomed Central

Specialties Biotechnology
Microbiology

Date 2003 Feb 28

PMID 12605725

Citations 33

Authors

Vivi Joosten

Christien Lokman

Cees Amjj van den Hondel

Peter J Punt

Affiliations

Soon will be listed here.

Abstract

In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody fragment production (scFv and VHH) by these lower eukaryotes and the possible applications of these proteins. Also the coupling of fragments to relevant enzymes or other components will be discussed. As an example of the fusion protein strategy, the 'magic bullet' approach for industrial applications, will be highlighted.

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