Genetic Diversity in Yeast Assessed with Whole-genome Oligonucleotide Arrays

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2003 Feb 15

PMID 12586698

Citations 81

Authors

Elizabeth A Winzeler

Cristian I Castillo-Davis

Guy Oshiro

David Liang

Daniel R Richards

Yingyao Zhou

Daniel L Hartl

Affiliations

Soon will be listed here.

Abstract

The availability of a complete genome sequence allows the detailed study of intraspecies variability. Here we use high-density oligonucleotide arrays to discover 11,115 single-feature polymorphisms (SFPs) existing in one or more of 14 different yeast strains. We use these SFPs to define regions of genetic identity between common laboratory strains of yeast. We assess the genome-wide distribution of genetic variation on the basis of this yeast population. We find that genome variability is biased toward the ends of chromosomes and is more likely to be found in genes with roles in fermentation or in transport. This subtelomeric bias may arise through recombination between nonhomologous sequences because full-gene deletions are more common in these regions than in more central regions of the chromosome.

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