Large-scale Functional Genomic Analysis of Sporulation and Meiosis in Saccharomyces Cerevisiae

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2003 Feb 15

PMID 12586695

Citations 135

Authors

Akon H Enyenihi

William S Saunders

Affiliations

Soon will be listed here.

Abstract

We have used a single-gene deletion mutant bank to identify the genes required for meiosis and sporulation among 4323 nonessential Saccharomyces cerevisiae annotated open reading frames (ORFs). Three hundred thirty-four sporulation-essential genes were identified, including 78 novel ORFs and 115 known genes without previously described sporulation defects in the comprehensive Saccharomyces Genome (SGD) or Yeast Proteome (YPD) phenotype databases. We have further divided the uncharacterized sporulation-essential genes into early, middle, and late stages of meiosis according to their requirement for IME1 induction and nuclear division. We believe this represents a nearly complete identification of the genes uniquely required for this complex cellular pathway. The set of genes identified in this phenotypic screen shows only limited overlap with those identified by expression-based studies.

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References

Primig M, Williams R, Winzeler E, Tevzadze G, Conway A, Hwang S . The core meiotic transcriptome in budding yeasts. Nat Genet. 2000; 26(4):415-23. DOI: 10.1038/82539. View

Jiao K, Bullard S, Salem L, Malone R . Coordination of the initiation of recombination and the reductional division in meiosis in Saccharomyces cerevisiae. Genetics. 1999; 152(1):117-28. PMC: 1460611. DOI: 10.1093/genetics/152.1.117. View

Briza P, Bogengruber E, Thur A, Rutzler M, Munsterkotter M, Dawes I . Systematic analysis of sporulation phenotypes in 624 non-lethal homozygous deletion strains of Saccharomyces cerevisiae. Yeast. 2002; 19(5):403-22. DOI: 10.1002/yea.843. View

Wu L, Hughes T, Davierwala A, Robinson M, Stoughton R, Altschuler S . Large-scale prediction of Saccharomyces cerevisiae gene function using overlapping transcriptional clusters. Nat Genet. 2002; 31(3):255-65. DOI: 10.1038/ng906. View

Giaever G, Chu A, Ni L, Connelly C, Riles L, Veronneau S . Functional profiling of the Saccharomyces cerevisiae genome. Nature. 2002; 418(6896):387-91. DOI: 10.1038/nature00935. View

Lynn R, Magee P . Development of the spore wall during ascospore formation in Saccharomyces cerevisiae. J Cell Biol. 1970; 44(3):688-92. PMC: 2107973. DOI: 10.1083/jcb.44.3.688. View

Moens P, Rapport E . Spindles, spindle plaques, and meiosis in the yeast Saccharomyces cerevisiae (Hansen). J Cell Biol. 1971; 50(2):344-61. PMC: 2108272. DOI: 10.1083/jcb.50.2.344. View

Dawes I, Hardie I . Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether. Mol Gen Genet. 1974; 131(4):281-9. DOI: 10.1007/BF00264859. View

Esposito M, Esposito R . Genes controlling meiosis and spore formation in yeast. Genetics. 1974; 78(1):215-25. PMC: 1213181. DOI: 10.1093/genetics/78.1.215. View

10.

Klapholz S, Waddell C, Esposito R . The role of the SPO11 gene in meiotic recombination in yeast. Genetics. 1985; 110(2):187-216. PMC: 1202559. DOI: 10.1093/genetics/110.2.187. View

11.

Kassir Y, Granot D, Simchen G . IME1, a positive regulator gene of meiosis in S. cerevisiae. Cell. 1988; 52(6):853-62. DOI: 10.1016/0092-8674(88)90427-8. View

12.

Rockmill B, Roeder G . RED1: a yeast gene required for the segregation of chromosomes during the reductional division of meiosis. Proc Natl Acad Sci U S A. 1988; 85(16):6057-61. PMC: 281904. DOI: 10.1073/pnas.85.16.6057. View

13.

Berben G, LEGRAIN M, Hilger F . Studies on the structure, expression and function of the yeast regulatory gene PHO2. Gene. 1988; 66(2):307-12. DOI: 10.1016/0378-1119(88)90367-8. View

14.

Smith H, Mitchell A . A transcriptional cascade governs entry into meiosis in Saccharomyces cerevisiae. Mol Cell Biol. 1989; 9(5):2142-52. PMC: 363008. DOI: 10.1128/mcb.9.5.2142-2152.1989. View

15.

Mitchell A, Driscoll S, Smith H . Positive control of sporulation-specific genes by the IME1 and IME2 products in Saccharomyces cerevisiae. Mol Cell Biol. 1990; 10(5):2104-10. PMC: 360558. DOI: 10.1128/mcb.10.5.2104-2110.1990. View

16.

Malone R . Dual regulation of meiosis in yeast. Cell. 1990; 61(3):375-8. DOI: 10.1016/0092-8674(90)90517-i. View

17.

Bartel B, Wunning I, Varshavsky A . The recognition component of the N-end rule pathway. EMBO J. 1990; 9(10):3179-89. PMC: 552047. DOI: 10.1002/j.1460-2075.1990.tb07516.x. View

18.

Briza P, Breitenbach M, Ellinger A, Segall J . Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae. Genes Dev. 1990; 4(10):1775-89. DOI: 10.1101/gad.4.10.1775. View

19.

Shah J, Clancy M . IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae. Mol Cell Biol. 1992; 12(3):1078-86. PMC: 369539. DOI: 10.1128/mcb.12.3.1078-1086.1992. View

20.

Hammond C, Romano P, Roe S, Tontonoz P . INO2, a regulatory gene in yeast phospholipid biosynthesis, affects nuclear segregation and bud pattern formation. Cell Mol Biol Res. 1993; 39(6):561-77. View