Rap1p and Other Transcriptional Regulators Can Function in Defining Distinct Domains of Gene Expression

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2003 Feb 13

PMID 12582242

Citations 12

Authors

Qun Yu

Runxiang Qiu

Travis B Foland

Dan Griesen

Carl S Galloway

Ya-Hui Chiu

Joseph Sandmeier

James R Broach

Xin Bi

Affiliations

Soon will be listed here.

Abstract

Barrier elements that are able to block the propagation of transcriptional silencing in yeast are functionally similar to chromatin boundary/insulator elements in metazoans that delimit functional chromosomal domains. We show that the upstream activating sequences of many highly expressed ribosome protein genes and glycolytic genes exhibit barrier activity. Analyses of these barriers indicate that binding sites for transcriptional regulators Rap1p, Abf1p, Reb1p, Adr1p and Gcn4p may participate in barrier function. We also present evidence suggesting that Rap1p is directly involved in barrier activity, and its barrier function correlates with local changes in chromatin structure. We further demonstrate that tethering the transcriptional activation domain of Rap1p to DNA is sufficient to recapitulate barrier activity. Moreover, targeting the activation domain of Adr1p or Gcn4p also establishes a barrier to silencing. These results support the notion that transcriptional regulators could also participate in delimiting functional domains in the genome.

Citing Articles

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Promoter architectures in the yeast ribosomal expression program.

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Highly transcribed RNA polymerase II genes are impediments to replication fork progression in Saccharomyces cerevisiae.

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