Individuality and Variation in Gene Expression Patterns in Human Blood

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2003 Feb 13

PMID 12578971

Citations 301

Authors

Adeline R Whitney

Maximilian Diehn

Stephen J Popper

Ash A Alizadeh

Jennifer C Boldrick

David A Relman

Patrick O Brown

Affiliations

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Abstract

The nature and extent of interindividual and temporal variation in gene expression patterns in specific cells and tissues is an important and relatively unexplored issue in human biology. We surveyed variation in gene expression patterns in peripheral blood from 75 healthy volunteers by using cDNA microarrays. Characterization of the variation in gene expression in healthy tissue is an essential foundation for the recognition and interpretation of the changes in these patterns associated with infections and other diseases, and peripheral blood was selected because it is a uniquely accessible tissue in which to examine this variation in patients or healthy volunteers in a clinical setting. Specific features of interindividual variation in gene expression patterns in peripheral blood could be traced to variation in the relative proportions of specific blood cell subsets; other features were correlated with gender, age, and the time of day at which the sample was taken. An analysis of multiple sequential samples from the same individuals allowed us to discern donor-specific patterns of gene expression. These data help to define human individuality and provide a database with which disease-associated gene expression patterns can be compared.

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