Recognition of Bovine Respiratory Syncytial Virus Proteins by Bovine CD8+ T Lymphocytes

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 2003 Feb 4

PMID 12562331

Citations 26

Authors

Ruth M Gaddum

Roy S Cook

Julie M Furze

Shirley A Ellis

Geraldine Taylor

Affiliations

Soon will be listed here.

Abstract

CD8+ T lymphocytes play a major role in the clearance of bovine respiratory syncytial virus (BRSV), an important respiratory pathogen of young calves that shares many of the epidemiological and pathological features of human respiratory syncytial virus (HRSV) in infants. Recombinant vaccinia virus (rVV) and recombinant fowlpox virus (rFPV), expressing individual BRSV proteins, were used to demonstrate that the F, N and M2 proteins were the major antigens recognized by bovine CD8+ T cells in major histocompatibility complex (MHC)-defined cattle. BRSV protein recognition by CD8+ T cells was analysed using cytotoxic T lymphocyte (CTL) assays or by the production of interferon-gamma (IFN-gamma) following restimulation with BRSV proteins. Strong recognition of the G protein by CD8+ T cells was observed in cattle that had been vaccinated with rVV expressing this protein and subsequently challenged with BRSV. Although there is variation in the number of expressed MHC genes in cattle with different class I haplotypes, this did not appear to influence BRSV protein recognition by CD8+ T cells. Knowledge of the antigenic specificity of BRSV-specific CD8+ T cells will facilitate the qualitative and quantitative analysis of BRSV-specific CD8+ T-cell memory in cattle and help to ensure that potential vaccines induce a qualitatively appropriate CD8+ T-cell response.

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References

Bangham C, Openshaw P, Ball L, King A, Wertz G, Askonas B . Human and murine cytotoxic T cells specific to respiratory syncytial virus recognize the viral nucleoprotein (N), but not the major glycoprotein (G), expressed by vaccinia virus recombinants. J Immunol. 1986; 137(12):3973-7. View

Collins R, Howard C, Duggan S, Werling D . Bovine interleukin-12 and modulation of IFNgamma production. Vet Immunol Immunopathol. 1999; 68(2-4):193-207. DOI: 10.1016/s0165-2427(99)00020-3. View

Cannon M, Openshaw P, Askonas B . Cytotoxic T cells clear virus but augment lung pathology in mice infected with respiratory syncytial virus. J Exp Med. 1988; 168(3):1163-8. PMC: 2189034. DOI: 10.1084/jem.168.3.1163. View

Lerch R, Stott E, Wertz G . Characterization of bovine respiratory syncytial virus proteins and mRNAs and generation of cDNA clones to the viral mRNAs. J Virol. 1989; 63(2):833-40. PMC: 247757. DOI: 10.1128/JVI.63.2.833-840.1989. View

Openshaw P, Anderson K, Wertz G, Askonas B . The 22,000-kilodalton protein of respiratory syncytial virus is a major target for Kd-restricted cytotoxic T lymphocytes from mice primed by infection. J Virol. 1990; 64(4):1683-9. PMC: 249305. DOI: 10.1128/JVI.64.4.1683-1689.1990. View

Lerch R, Anderson K, Wertz G . Nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment protein G of bovine respiratory syncytial virus is distinct from that of human respiratory syncytial virus. J Virol. 1990; 64(11):5559-69. PMC: 248608. DOI: 10.1128/JVI.64.11.5559-5569.1990. View

Lerch R, Anderson K, Amann V, Wertz G . Nucleotide sequence analysis of the bovine respiratory syncytial virus fusion protein mRNA and expression from a recombinant vaccinia virus. Virology. 1991; 181(1):118-31. DOI: 10.1016/0042-6822(91)90476-r. View

Connors M, Collins P, Firestone C, Murphy B . Respiratory syncytial virus (RSV) F, G, M2 (22K), and N proteins each induce resistance to RSV challenge, but resistance induced by M2 and N proteins is relatively short-lived. J Virol. 1991; 65(3):1634-7. PMC: 239952. DOI: 10.1128/JVI.65.3.1634-1637.1991. View

Ellis S, Holmes E, Staines K, Smith K, Stear M, McKeever D . Variation in the number of expressed MHC genes in different cattle class I haplotypes. Immunogenetics. 2000; 50(5-6):319-28. DOI: 10.1007/s002510050608. View

10.

Goulder P, Lechner F, Klenerman P, McIntosh K, Walker B . Characterization of a novel respiratory syncytial virus-specific human cytotoxic T-lymphocyte epitope. J Virol. 2000; 74(16):7694-7. PMC: 112296. DOI: 10.1128/jvi.74.16.7694-7697.2000. View

11.

Brandenburg A, de Waal L, Timmerman H, Hoogerhout P, de Swart R, Osterhaus A . HLA class I-restricted cytotoxic T-cell epitopes of the respiratory syncytial virus fusion protein. J Virol. 2000; 74(21):10240-4. PMC: 102066. DOI: 10.1128/jvi.74.21.10240-10244.2000. View

12.

Schreiber P, Matheise J, Dessy F, Heimann M, Letesson J, Coppe P . High mortality rate associated with bovine respiratory syncytial virus (BRSV) infection in Belgian white blue calves previously vaccinated with an inactivated BRSV vaccine. J Vet Med B Infect Dis Vet Public Health. 2000; 47(7):535-50. DOI: 10.1046/j.1439-0450.2000.00380.x. View

13.

Chang J, Srikiatkhachorn A, Braciale T . Visualization and characterization of respiratory syncytial virus F-specific CD8(+) T cells during experimental virus infection. J Immunol. 2001; 167(8):4254-60. DOI: 10.4049/jimmunol.167.8.4254. View

14.

Chang J, Braciale T . Respiratory syncytial virus infection suppresses lung CD8+ T-cell effector activity and peripheral CD8+ T-cell memory in the respiratory tract. Nat Med. 2002; 8(1):54-60. DOI: 10.1038/nm0102-54. View

15.

Hope J, Sopp P, Howard C . NK-like CD8(+) cells in immunologically naïve neonatal calves that respond to dendritic cells infected with Mycobacterium bovis BCG. J Leukoc Biol. 2002; 71(2):184-94. View

16.

Kim H, Canchola J, BRANDT C, Pyles G, Chanock R, Jensen K . Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. Am J Epidemiol. 1969; 89(4):422-34. DOI: 10.1093/oxfordjournals.aje.a120955. View

17.

Zaia J, Oxman M . Antibody to varicella-zoster virus-induced membrane antigen: immunofluorescence assay using monodisperse glutaraldehyde-fixed target cells. J Infect Dis. 1977; 136(4):519-30. DOI: 10.1093/infdis/136.4.519. View

18.

Thomas L, GOURLAY R, Stott E, Howard C, Bridger J . A search for new microorganisms in calf pneumonia by the inoculation of gnotobiotic calves. Res Vet Sci. 1982; 33(2):170-82. PMC: 7126884. View

19.

Taylor G, Stott E, BEW M, Fernie B, Cote P, Collins A . Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology. 1984; 52(1):137-42. PMC: 1454581. View

20.

Stott E, Thomas L, Taylor G, Collins A, Jebbett J, Crouch S . A comparison of three vaccines against respiratory syncytial virus in calves. J Hyg (Lond). 1984; 93(2):251-61. PMC: 2129425. DOI: 10.1017/s0022172400064779. View