Process of Infection with Bacteriophage Phi X 174 XXXVIII. Replication of Phi Chi 174 Replicative Form in Vivo

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1976 Mar 1

PMID 1255857

Citations 3

Authors

A Fukuda

R L SINSHEIMER

Affiliations

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Abstract

The replication of bacteriophage phi X 174 replicative-form DNA has been studied by structural analysis of pulse-labeled replicative-intermediate molecules. Such intermediates were identified by pulse-labeling with [13H]thymidine and separated into four major fractions (A, B, C, and D) in a propidium diiodide-cesium chloride buoyand density gradient. Sedimentation analysis of each of these fractions suggests the following features of phi X replicative-form DNA replication in vivo. (i) At the end of one cycle of replication, one daughter replicative form (RFII) contains a nascent plus (+) strand of the unit viral length, and the other daughter RFII contains small fragments of nascent minus (-) strand. (ii) Asymmetry is also associated with production of the first supercoiled RFI after addition of pulse label in that only the minus strand becomes radioactive. (iii) A supercoiled DNA (RFI') seems to occur in vivo. This DNA is observed at a position of greater density in a propidium diiodide-cesium chloride buoyant density gradient than normal RFI. (iv) A novel DNA component is observed, at a density greater than RFI, which releases, in alkali, a plus strand longer (1.5 to 1.7 times) than the unit viral length. These results are discussed in terms of the possible sequence of events in phi X 174 replicative-form replication in vivo.

Citing Articles

Enzymatic replication of viral and complementary strands of duplex DNA of phage phiX174 proceeds by seprate mechanisms.

Eisenberg S, Scott J, Kornberg A Proc Natl Acad Sci U S A. 1976; 73(9):3151-5.

PMID: 1067608 PMC: 430963. DOI: 10.1073/pnas.73.9.3151.

Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA.

Ikeda J, Yudelevich A, Hurwitz J Proc Natl Acad Sci U S A. 1976; 73(8):2669-73.

PMID: 1066678 PMC: 430709. DOI: 10.1073/pnas.73.8.2669.

Discontinuous replication of replicative form DNA from bacteriophage phiX174.

Machida Y, Okazaki T, Okazaki R Proc Natl Acad Sci U S A. 1977; 74(7):2776-9.

PMID: 268627 PMC: 431286. DOI: 10.1073/pnas.74.7.2776.

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