Optical Cell with a Temperature-control Unit for a Vacuum-ultraviolet Circular Dichroism Spectrophotometer

Overview

Journal Anal Sci

Publisher Springer

Specialty Chemistry

Date 2003 Feb 1

PMID 12558036

Citations 11

Authors

Koichi Matsuo

Kenichi Sakai

Yosuke Matsushima

Takayuki Fukuyama

Kunihiko Gekko

Affiliations

Soon will be listed here.

Abstract

We constructed an assembled-type MgF2 cell that can function under a high vacuum (10(-4) Pa), and is capable of measuring the vacuum-ultraviolet circular dichroism (VUVCD) spectrum in a wavelength region down to 140 nm for aqueous solutions. Its path length can be adjusted by various spacers in the range from 1.3 to 50 microm. The temperature-control unit of the cell was also constructed with a Peltier thermoelectric element to keep the temperature of a sample within an accuracy of +/- 1 degrees C in the temperature range from -30 to 70 degrees C. The optical cell and the temperature-control unit were confirmed to have good performance by monitoring the VUVCD spectra of ammonium d-camphor-10-sulfonate and myoglobin aqueous solutions. This cell is available not only for VUVCD spectroscopy, but also for vacuum-ultraviolet absorption measurements.

Citing Articles

Mechanistic Insights Into Oxidative Response of Heat Shock Factor 1 Condensates.

Kawagoe S, Matsusaki M, Mabuchi T, Ogasawara Y, Watanabe K, Ishimori K JACS Au. 2025; 5(2):606-617.

PMID: 40017748 PMC: 11863153. DOI: 10.1021/jacsau.4c00578.

Potential Bioactivities, Chemical Composition, and Conformation Studies of Exopolysaccharide-Derived sp. Strain GAD7.

Ibrahim M, Ibrahim H, Haga T, Ishida A, Nehira T, Matsuo K J Fungi (Basel). 2024; 10(9).

PMID: 39330418 PMC: 11432975. DOI: 10.3390/jof10090659.

Formation of β-Strand Oligomers of Antimicrobial Peptide Magainin 2 Contributes to Disruption of Phospholipid Membrane.

Kumashiro M, Tsuji R, Suenaga S, Matsuo K Membranes (Basel). 2022; 12(2).

PMID: 35207051 PMC: 8877076. DOI: 10.3390/membranes12020131.

Regulation of plant ER oxidoreductin 1 (ERO1) activity for efficient oxidative protein folding.

Matsusaki M, Okuda A, Matsuo K, Gekko K, Masuda T, Naruo Y J Biol Chem. 2019; 294(49):18820-18835.

PMID: 31685660 PMC: 6901294. DOI: 10.1074/jbc.RA119.010917.

Sample Volume Reduction Using the Schwarzschild Objective for a Circular Dichroism Spectrophotometer and an Application to the Structural Analysis of Lysine-36 Trimethylated Histone H3 Protein.

Izumi Y, Matsuo K Molecules. 2018; 23(11).

PMID: 30400257 PMC: 6278440. DOI: 10.3390/molecules23112865.