The Effect of Thiols on Saccharomyces Fragilis

Overview

Journal Antonie Van Leeuwenhoek

Publisher Springer

Specialty Microbiology

Date 1975 Jan 1

PMID 125559

Citations 3

Authors

R Davies

F J Wayman

Affiliations

Soon will be listed here.

Abstract

Treatment of cell suspensions of Saccharomyces fragilis with 0.01 M beta-mercaptoethanol or dithiothreitol released a variety of substances of high and low molecular weight. Twenty-two high-molecular-weight glycoproteins were separated by a combination of chromatography on DEAE cellulose and polyacrylamide gel electrophoresis in presence of sodium dodecylsulphate. The carbohydrate components consisted of at least 95% mannose and the protein components had threonine and serine as the major amino acids. Only very small amounts of phosphorus were associated with the high-molecular-weight components. The low-molecular-weight substances werr probably released from the internal cell pool and uracil and hypoxanthine were identified as components of this fraction. It is suggested that in addition to breaking disulphide bridges in the cell wall the thiols may also render the plasmalemma permeable to certain low-molecular-weight substances. Such effects are not lethal since the yeast can be trained to grow in presence of 0.01 M mercaptoethanol.

Citing Articles

Localization of inulinase and invertase in Kluyveromyces species.

Rouwenhorst R, Ritmeester W, Scheffers W, van Dijken J Appl Environ Microbiol. 1990; 56(11):3329-36.

PMID: 2268150 PMC: 184950. DOI: 10.1128/aem.56.11.3329-3336.1990.

Structure and properties of the extracellular inulinase of Kluyveromyces marxianus CBS 6556.

Rouwenhorst R, Hensing M, Verbakel J, Scheffers W, van Duken J Appl Environ Microbiol. 1990; 56(11):3337-45.

PMID: 2135869 PMC: 184951. DOI: 10.1128/aem.56.11.3337-3345.1990.

Production and localization of beta-fructosidase in asynchronous and synchronous chemostat cultures of yeasts.

Rouwenhorst R, Van Der Baan A, Scheffers W, van Dijken J Appl Environ Microbiol. 1991; 57(2):557-62.

PMID: 2014991 PMC: 182748. DOI: 10.1128/aem.57.2.557-562.1991.

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