Heterogeneity of Human Cell Membrane Sialoglycoproteins

Overview

Journal Blut

Specialty Hematology

Date 1976 Mar 1

PMID 1252623

Citations 15

Authors

W Dahr

G Uhlenbruck

E Janssen

R Schmalisch

Affiliations

Soon will be listed here.

Abstract

Discontinuous sodium dodecysulfate polyacrylamide gel electrophoresis (disc SDS-PAGE) followed by periodic acid/Schiff staining reveals the presence of six sialoglycoprotein bands in human red cell membranes or glycoprotein preparations therefrom. In agreement with previous investigations it is shown that PAS-1 and PAS-2 (mol. weight 37 000) are different forms of the same molecule (MN glycoprotein). Using separation of glycoproteins by the system of Weber and Osborn and reelectrophoresis of gel slices by disc SDS-PAGE it is demonstrated that the minor component C (mol. weight 41 000) represents the dimeric form of PAS-3 (Ss glycoprotein). Band B corresponds to an aggregate of PAS-3 and PAS-2 and/or the trimer of PAS-3 with possible differences between extracted glycoproteins and those present in the membrane. The minor component D (mol. weight 35 000) is, as far as could be elucidated, not involved in aggregation phenomena. Some technical problems of glycoprotein fractionation by SDS-PAGE and the remarkable effect of phosphate buffers on the glycoprotein pattern are discussed.

Citing Articles

Cervical spinal cord injury leads to injury and altered metabolism in the lungs.

Huffman E, Dong B, Clarke H, Young L, Gentry M, Allison D Brain Commun. 2023; 5(2):fcad091.

PMID: 37065091 PMC: 10090796. DOI: 10.1093/braincomms/fcad091.

High thermal amplitude auto-anti-"N" complicating pretransfusion compatibility tests on a patient with malaria and bladder cancer.

Joshi S, Sheladiya A, Agrawal V Asian J Transfus Sci. 2019; 13(1):30-33.

PMID: 31360007 PMC: 6580829. DOI: 10.4103/ajts.AJTS_92_18.

Clinical Features, Survival and Prognostic Factors of Glycogen-Rich Clear Cell Carcinoma (GRCC) of the Breast in the U.S. Population.

Zhou Z, Kinslow C, Hibshoosh H, Guo H, Cheng S, He C J Clin Med. 2019; 8(2).

PMID: 30769905 PMC: 6406344. DOI: 10.3390/jcm8020246.

Novel method for detection of glycogen in cells.

Skurat A, Segvich D, DePaoli-Roach A, Roach P Glycobiology. 2017; 27(5):416-424.

PMID: 28077463 PMC: 5444244. DOI: 10.1093/glycob/cwx005.

Construction of recombinant hemagglutinin derived from the gingipain-encoding gene of Porphyromonas gingivalis, identification of its target protein on erythrocytes, and inhibition of hemagglutination by an interdomain regional peptide.

Sakai E, Naito M, Sato K, Hotokezaka H, Kadowaki T, Kamaguchi A J Bacteriol. 2007; 189(11):3977-86.

PMID: 17384191 PMC: 1913415. DOI: 10.1128/JB.01691-06.

References

Habeeb A . Determination of free amino groups in proteins by trinitrobenzenesulfonic acid. Anal Biochem. 1966; 14(3):328-36. DOI: 10.1016/0003-2697(66)90275-2. View

Azuma J, JANADO M, Onodera K . Heterogeneity of a human erythrocyte membrane glycoprotein. J Biochem. 1973; 73(5):1127-30. DOI: 10.1093/oxfordjournals.jbchem.a130170. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Fairbanks G, Steck T, Wallach D . Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971; 10(13):2606-17. DOI: 10.1021/bi00789a030. View

Dahr W, Uhlenbruck G, Gunson H, van der Hart M . Molecular basis of Tn-polyagglutinability. Vox Sang. 1975; 29(1):36-50. DOI: 10.1111/j.1423-0410.1975.tb00475.x. View

Segrest J, Jackson R, Andrews E, Marchesi V . Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis. Biochem Biophys Res Commun. 1971; 44(2):390-5. DOI: 10.1016/0006-291x(71)90612-7. View

Tuech J, Morrison M . Human erythrocyte membrane sialoglycoproteins: a study of interconversion. Biochem Biophys Res Commun. 1974; 59(1):352-60. DOI: 10.1016/s0006-291x(74)80214-7. View

Dahr W, Uhlenbruck G, Bird G . Cryptic A-like receptor sites in human erythrocyte glycoproteins: proposed nature of Tn-antigen. Vox Sang. 1974; 27(1):29-42. DOI: 10.1111/j.1423-0410.1974.tb02386.x. View

Furthmayr H, Tomita M, Marchesi V . Fractionation of the major sialoglycopeptides of the human red blood cell membrane. Biochem Biophys Res Commun. 1975; 65(1):113-21. DOI: 10.1016/s0006-291x(75)80068-4. View

10.

Dahr W, Uhlenbruck G, Bird G . Influence of free amino and carboxyl groups on the specificity of plant anti-N. Vox Sang. 1975; 28(5):389-91. DOI: 10.1111/j.1423-0410.1975.tb02785.x. View

11.

Lea E, RICH G, Segrest J . The effects of the membrane-penetrating polypeptide segment of the human erythrocyte MN-glycoprotein on the permeability of model lipid membranes. Biochim Biophys Acta. 1975; 382(1):41-50. DOI: 10.1016/0005-2736(75)90370-3. View

12.

Dahr W, Uhlenbruck G, Schmalisch R, Janssen E . Ss blood group associated PAS-staining polymorphism of glycoprotein 3 from human erythrocyte membranes. Hum Genet. 1976; 32(2):121-32. DOI: 10.1007/BF00291494. View

13.

Uhlenbruck G, Rothe A, PARDOE G . [Noteworthy chemical, serologic and electrokinetic phenomena in cat erythrocytes: a contrtibution to the determination and importance of mucoid and glycolipoid bound erythrocyte neuraminic acid]. Z Immunitatsforsch Allerg Klin Immunol. 1968; 136(1):79-97. View

14.

Eshhar Z, Gafni M, Givol D, Sela M . Solubilization of lymphocyte and thymocyte antigens by a reversible chemical modification. Eur J Immunol. 1971; 1(5):323-9. DOI: 10.1002/eji.1830010504. View

15.

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View

16.

Lisowska E, MORAWIECKI A . The role of free amino groups in the blood group activity of M and N mucoids. Eur J Biochem. 1967; 3(2):237-41. DOI: 10.1111/j.1432-1033.1967.tb19522.x. View

17.

Hamaguchi H, CLEVE H . Solubilization of human erythrocyte membrane glycoproteins and separation of the MN glycoprotein from a glycoprotein with I, S, and A activity. Biochim Biophys Acta. 1972; 278(2):271-80. DOI: 10.1016/0005-2795(72)90232-2. View

18.

Fujita S, CLEVE H . Isolation and partial characterization of two minor glycoproteins from human erythrocyte membranes. Biochim Biophys Acta. 1975; 382(2):172-80. DOI: 10.1016/0005-2736(75)90175-3. View

19.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

20.

Fukuda M, Osawa T . Isolation and characterization of a glycoprotein from human group O erythrocyte membrane. J Biol Chem. 1973; 248(14):5100-5. View