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Effect of Multiple Freeze-thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies

Overview
Journal Clin Diagn Lab Immunol
Publisher American Society for Microbiology
Specialty Allergy & Immunology
Date 2003 Jan 11
PMID 12522034
Citations 24
Authors
Norman A Pinsky
Jeanne M Huddleston
Robert M Jacobson
Peter C Wollan
Gregory A Poland
Affiliations
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Abstract

We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4 degrees C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.

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