Biochemical Characterization of a Mutationally Altered Protein Translocase: Proton Motive Force Stimulation of the Initiation Phase of Translocation

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2003 Jan 4

PMID 12511485

Citations 14

Authors

Hiroyuki Mori

Koreaki Ito

Affiliations

Soon will be listed here.

Abstract

Protein translocation across the Escherichia coli plasma membrane is facilitated by concerted actions of the SecYEG integral membrane complex and the SecA ATPase. A secY mutation (secY39) affects Arg357, an evolutionarily conserved and functionally important residue, and impairs the translocation function in vivo and in vitro. In this study, we used the "superactive" mutant forms of SecA, which suppress the SecY39 deficiency, to characterize the mutationally altered SecY39EG translocase. It was found that SecY39-mediated preprotein translocation exhibited absolute dependence on the proton motive force. The proton motive force-dependent step proved to lie before signal peptide cleavage. We suggest that the proton motive force assists in the initiation phase of protein translocation.

Citing Articles

The Dynamic SecYEG Translocon.

Oswald J, Njenga R, Natriashvili A, Sarmah P, Koch H Front Mol Biosci. 2021; 8:664241.

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SecA-Mediated Protein Translocation through the SecYEG Channel.

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An energy transduction mechanism used in bacterial flagellar type III protein export.

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Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria.

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Visualization of distinct entities of the SecYEG translocon during translocation and integration of bacterial proteins.

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