Phosphorus Nuclear Magnetic Resonance Studies on Normoxic and Ischemic Cardiac Tissue

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 Dec 1

PMID 12507

Citations 19

Authors

D G Gadian

D I Hoult

G K Radda

P J Seeley

B Chance

C Barlow

Affiliations

Soon will be listed here.

Abstract

The intact heart of a young rat was excised rapidly and cooled to 0 degree C; its energy-rich compounds were examined by 31P Fourier Transform nuclear magnetic resonance. The heart showed the characteristic spectrum of sugar phosphates, inorganic phosphate, phosphocreatine, and magniesium phates, inorganic phosphate, phosphocreatine, and magnesium ATP, characteristics of the energizing state of the nonbeating tissue. Warming to 30 degrees C imposes an energy load upon the heart consistent with short-term resumption of beating, concomitant intracellular acidosis, and decomposition of all detectable energy-rich compounds. The intracellular acidity causes a shift from pH 7.0 to 6.0. The effects of possible interferences with this pH measurement are considered. The method appears to have wide usefulness in cardiac infarct models for detecting the fraction of the total volume occupied by the infarct and for studying the effect of various proposed therapies upon this infarcted volume.

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References

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Hoult D, Busby S, Gadian D, Radda G, Richards R, Seeley P . Observation of tissue metabolites using 31P nuclear magnetic resonance. Nature. 1974; 252(5481):285-7. DOI: 10.1038/252285a0. View

Burt C, Glonek T, Barany M . Analysis of phosphate metabolites, the intracellular pH, and the state of adenosine triphosphate in intact muscle by phosphorus nuclear magnetic resonance. J Biol Chem. 1976; 251(9):2584-91. View