Spinning-disk Confocal Microscopy -- a Cutting-edge Tool for Imaging of Membrane Traffic

Overview

Journal Cell Struct Funct

Specialty Cell Biology

Date 2002 Dec 28

PMID 12502889

Citations 53

Authors

Akihiko Nakano

Affiliations

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Abstract

Confocal laser scanning microscopy is experiencing a revolution in speed from the world of seconds to that of milliseconds. The spinning Nipkow disk method with microlenses has made this remarkable innovation possible. In combination with the ultrahigh-sensitivity, high-speed and high-resolution camera system based on avalanche multiplication of photoconduction, we are now able to observe the extremely dynamic movement of small vesicles in living cells in real time.

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