Association of Helicobacter Pylori Antioxidant Activities with Host Colonization Proficiency

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2002 Dec 24

PMID 12496216

Citations 32

Authors

Adriana A Olczak

Richard W Seyler Jr

Jonathan W Olson

Robert J Maier

Affiliations

Soon will be listed here.

Abstract

To assess the importance of two separate antioxidant activities in Helicobacter pylori, we tested the abilities of strains with mutations in either tpx (encoding thiolperoxidase) or ahpC (encoding alkyl hydroperoxide reductase [AhpC]) to colonize the stomachs of mice. The tpx strain was clearly more sensitive than the parent strain to both oxygen and cumene hydroperoxide. The strain colonized only 5% of the inoculated mice. Two different classes of oxygen-sensitive ahpC mutants in the type strain (ATCC 43504) were recently described (A. A. Olczak, J. W. Olson, and R. J. Maier, J. Bacteriol. 184:3186-3193, 2002). The same two classes of mutants were recovered upon ahpC mutagenesis of the mouse-adapted strain, SS1. Neither of these mutants was able to colonize mouse stomachs, whereas 78% of the mice inoculated with the parent strain became H. pylori positive.

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