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Mapping of the Chromosome of Mycobacterium Phlei by Means of Mutagenesis of the Replication Point

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Publisher Springer
Specialty Microbiology
Date 1976 Jan 1
PMID 1248781
Citations 7
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Abstract

The aim of the present work was to construct a replication map of the chromosome of Mycobacterium phlei. The method of mutagenesis of the replication point by means of nitrosoguanidine was applied to synchronously multiplying populations. Back mutations and forward mutations were induced in auxotrophic mutants PA met and PA leu as well as in double auxotrophic mutants with methionine as the reference marker and the following order of replication of eleven genes on the chromosome was thus established: leu-Eth, Res-Stm, Cyk-pur-met, arg, Cyk-Bac-inl.

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Mutagenic effect of n-methyl-N-nitrosourea on Mycobacterium phlei.

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The completion of the replication map of the Mycobacterium phlei chromosome.

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Mycobacteria in the light of modern genetics development.

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Gene manipulation in mycobacteria.

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