In Saccharomyces Cerevisiae, ATP2 MRNA Sorting to the Vicinity of Mitochondria is Essential for Respiratory Function

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2002 Dec 18

PMID 12486010

Citations 47

Authors

Antoine Margeot

Corinne Blugeon

Julien Sylvestre

Stephane Vialette

Claude Jacq

Marisol Corral-Debrinski

Affiliations

Soon will be listed here.

Abstract

We recently demonstrated that polysome-associated mRNAs that co-isolate with mitochondria encode a subset of mitochondrial proteins, and that the 3' UTRs of these transcripts are essential for their localization to the vicinity of the organelle. To address the question of the involvement of the mRNA targeting process in mitochondrial biogenesis, we studied the role of ATP2 3' UTR. An altered ATP2 allele in which the 3' UTR was replaced by the ADH1 3' UTR exhibits properties supporting the importance of mRNA localization to the vicinity of mitochondria: (i) the mutated strain presents a respiratory dysfunction; (ii) mitochondrial import of the protein translated from the altered gene is strongly reduced, even though the precursor is addressed to the organelle surface; (iii) systematic deletions of ATP2 3' UTR revealed a 100 nucleotide element presenting RNA targeting properties. Additionally, when the ATM1 3' UTR was replaced by the ADH1 3' UTR, we obtained cells in which ATM1 mRNA is also delocalized, and presenting a respiratory dysfunction. This demonstrates that mRNA localization to the vicinity of mitochondria plays a critical role in organelle biogenesis.

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