Delayed Reentry of Recycling Vesicles into the Fusion-competent Synaptic Vesicle Pool in Synaptojanin 1 Knockout Mice

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 Dec 14

PMID 12481038

Citations 61

Authors

Warren T Kim

Sunghoe Chang

Laurie Daniell

Ottavio Cremona

Gilbert Di Paolo

Pietro De Camilli

Affiliations

Soon will be listed here.

Abstract

Synaptojanin 1 is a polyphosphoinositide phosphatase implicated in synaptic vesicle recycling. We used FM1-43 imaging and electron microscopy in cultured cortical neurons from control and synaptojanin 1 knockout mice to study how the absence of this protein affects specific steps of the synaptic vesicle cycle. Exoendocytosis after a moderate stimulus was unchanged. However, during prolonged stimulation, the regeneration of fusion-competent synaptic vesicles was severely impaired. In stimulated nerve terminals, there was a persistent accumulation of clathrin-coated vesicles and a backup of newly reformed vesicles in the cytomatrix-rich area around the synaptic vesicle cluster. These findings demonstrate that synaptojanin 1 function is needed for the progression of recycling vesicles to the functional synaptic vesicle pool.

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