Interorganellar Regulation of Lysosome Positioning by the Golgi Apparatus Through Rab34 Interaction with Rab-interacting Lysosomal Protein

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2002 Dec 12

PMID 12475955

Citations 66

Authors

Tuanlao Wang

Wanjin Hong

Affiliations

Soon will be listed here.

Abstract

We present evidence to suggest the existence of a regulatory pathway for the Golgi apparatus to modulate the spatial positioning of otherwise distantly located lysosomes. Rab34, a new member of the Rab GTPase family, is associated primarily with the Golgi apparatus. Expression of wild-type or GTP-restricted but not GDP-restricted versions of Rab34 causes spatial redistribution of lysosomes from the periphery to the peri-Golgi region. The regulation of lysosomal positioning by Rab34 depends on its association with the membrane mediated by prenylation and its direct interaction with Rab-interacting lysosomal protein (RILP). This biological activity, mediated by Rab34-RILP interaction, is dependent on Lys82 in the switch I region. Our results have uncovered a novel mechanism for the Golgi apparatus to regulate the spatial distribution of another organelle.

Citing Articles

RILP Induces Cholesterol Accumulation in Lysosomes by Inhibiting Endoplasmic Reticulum-Endolysosome Interactions.

Han Y, Liu X, Xu L, Wei Z, Gu Y, Ren Y Cells. 2024; 13(16.

PMID: 39195203 PMC: 11352460. DOI: 10.3390/cells13161313.

Biogenesis of specialized lysosomes in differentiated keratinocytes relies on close apposition with the Golgi apparatus.

Mahanty S, Bergam P, Belapurkar V, Eluvathingal L, Gupta N, Goud B Cell Death Dis. 2024; 15(7):496.

PMID: 38992005 PMC: 11239851. DOI: 10.1038/s41419-024-06710-w.

-containing vacuoles interact with host recycling endosomal proteins Rab11a and Rab35 for vacuolar expansion and bacterial growth.

Hall B, Senior K, Ocampo N, Samanta D Front Cell Infect Microbiol. 2024; 14:1394019.

PMID: 38841112 PMC: 11150555. DOI: 10.3389/fcimb.2024.1394019.

Functional Divergence of the Paralog Effector Proteins SopD and SopD2 and Their Contributions to Infection.

Oke M, DCosta V Int J Mol Sci. 2024; 25(8).

PMID: 38673776 PMC: 11050076. DOI: 10.3390/ijms25084191.

Altered TFEB subcellular localization in nigral neurons of subjects with incidental, sporadic and GBA-related Lewy body diseases.

Moors T, Morella M, Bertran-Cobo C, Geut H, Udayar V, Timmermans-Huisman E Acta Neuropathol. 2024; 147(1):67.

PMID: 38581586 PMC: 10998821. DOI: 10.1007/s00401-024-02707-z.

References

Allan B, Moyer B, Balch W . Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion. Science. 2000; 289(5478):444-8. DOI: 10.1126/science.289.5478.444. View

Zhu X, Boman A, Kuai J, Cieplak W, Kahn R . Effectors increase the affinity of ADP-ribosylation factor for GTP to increase binding. J Biol Chem. 2000; 275(18):13465-75. DOI: 10.1074/jbc.275.18.13465. View

Short B, Preisinger C, Korner R, Kopajtich R, Byron O, Barr F . A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic. J Cell Biol. 2001; 155(6):877-83. PMC: 2150909. DOI: 10.1083/jcb.200108079. View

Xu Y, Hortsman H, Seet L, Wong S, Hong W . SNX3 regulates endosomal function through its PX-domain-mediated interaction with PtdIns(3)P. Nat Cell Biol. 2001; 3(7):658-66. DOI: 10.1038/35083051. View

Echard A, Opdam F, de Leeuw H, Jollivet F, Savelkoul P, Hendriks W . Alternative splicing of the human Rab6A gene generates two close but functionally different isoforms. Mol Biol Cell. 2000; 11(11):3819-33. PMC: 15039. DOI: 10.1091/mbc.11.11.3819. View

Chavrier P, Goud B . The role of ARF and Rab GTPases in membrane transport. Curr Opin Cell Biol. 1999; 11(4):466-75. DOI: 10.1016/S0955-0674(99)80067-2. View

Perou C, LESLIE J, Green W, Li L, Ward D, Kaplan J . The Beige/Chediak-Higashi syndrome gene encodes a widely expressed cytosolic protein. J Biol Chem. 1997; 272(47):29790-4. DOI: 10.1074/jbc.272.47.29790. View

Hayles J, Nurse P . A journey into space. Nat Rev Mol Cell Biol. 2001; 2(9):647-56. DOI: 10.1038/35089520. View

Gu F, Crump C, Thomas G . Trans-Golgi network sorting. Cell Mol Life Sci. 2001; 58(8):1067-84. PMC: 1424219. DOI: 10.1007/PL00000922. View

10.

Pereira-Leal J, Seabra M . Evolution of the Rab family of small GTP-binding proteins. J Mol Biol. 2001; 313(4):889-901. DOI: 10.1006/jmbi.2001.5072. View

11.

Marceiller J, Perez F, Settegrana C, Drechou A, Durand G, Pous C . The Golgi complex is a microtubule-organizing organelle. Mol Biol Cell. 2001; 12(7):2047-60. PMC: 55652. DOI: 10.1091/mbc.12.7.2047. View

12.

Tisdale E, Balch W . Rab2 is essential for the maturation of pre-Golgi intermediates. J Biol Chem. 1996; 271(46):29372-9. DOI: 10.1074/jbc.271.46.29372. View

13.

Darchen F, Goud B . Multiple aspects of Rab protein action in the secretory pathway: focus on Rab3 and Rab6. Biochimie. 2000; 82(4):375-84. DOI: 10.1016/s0300-9084(00)00219-4. View

14.

Moyer B, Allan B, Balch W . Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis--Golgi tethering. Traffic. 2001; 2(4):268-76. DOI: 10.1034/j.1600-0854.2001.1o007.x. View

15.

Hirokawa N . Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science. 1998; 279(5350):519-26. DOI: 10.1126/science.279.5350.519. View

16.

Lippincott-Schwartz J . Cytoskeletal proteins and Golgi dynamics. Curr Opin Cell Biol. 1998; 10(1):52-9. DOI: 10.1016/s0955-0674(98)80086-0. View

17.

Segev N . Ypt and Rab GTPases: insight into functions through novel interactions. Curr Opin Cell Biol. 2001; 13(4):500-11. DOI: 10.1016/s0955-0674(00)00242-8. View

18.

Knoblich J . Asymmetric cell division during animal development. Nat Rev Mol Cell Biol. 2001; 2(1):11-20. DOI: 10.1038/35048085. View

19.

Barr F . A novel Rab6-interacting domain defines a family of Golgi-targeted coiled-coil proteins. Curr Biol. 1999; 9(7):381-4. DOI: 10.1016/s0960-9822(99)80167-5. View

20.

Karcher R, Deacon S, Gelfand V . Motor-cargo interactions: the key to transport specificity. Trends Cell Biol. 2002; 12(1):21-7. DOI: 10.1016/s0962-8924(01)02184-5. View